Comment[ArrayExpressAccession]	E-MTAB-1469
Investigation Title	Comparative analysis of Mbl attenuation and CUG non-coding repeats expression in Drosophila larval muscles
Comment[Submitted Name]	Comparative analysis of Mbl attenuation and CUG non-coding repeats expression in Drosophila larval muscles
Experiment Description	Myotonic dystrophy type 1 (DM1) is a neuro-muscular disorder caused by CTG triplet expansion in the 3-UTR of the DMPK gene. Mutated transcripts aggregate in muscle nuclei and sequester the MBNL1 splicing factor. To assess the involvement of Mbl sequestration on transcriptpion deregulation in DM1, we performed genome wide analyses of gene expression of DM1 Drosophila model in three different genetic contexts: Mef>mblRNAi, Mef>600CTG, Mef>960CTG vs. Mef>lacZ control line.
Experimental Design	disease_state_design	reference_design	co-expression_design
Comment[AEExperimentType]	transcription profiling by array
Comment[AEExperimentDisplayName]	Transcription profiling by array of Myotonic dystrophy type 1 (DM1) Drosophila model in three different genetic contexts to assess the involvement of Mbl sequestration on transcriptpion deregulation in DM1
Experimental Factor Name	DISEASE_STATE	GENOTYPE
Experimental Factor Type	disease	genotype
Quality Control Type	biological replicate
Public Release Date	2013-04-01
Person Last Name	Picchio
Person First Name	Lucie
Person Mid Initials
Person Email	luciepicchio@yahoo.fr
Person Phone
Person Address	Clermont-Ferrand, France
Person Affiliation	GReD (Genetics, Reproduction and Development laboratory), INSERM 1103, CNRS 6293, University of Clermont-Ferrand
Person Roles	submitter; investigator
PubMed ID	23525904
Publication Author List	Lucie Picchio; Emilie Plantie; Yoan Renaud; Preethi Poovthumkadavil;
Krzysztof Jagla
Publication Title	Novel Drosophila model of myotonic dystrophy type 1: phenotypic characterization
and genome-wide view of altered gene expression
Publication Status	submitted
Protocol Name	P-MTAB-30772	P-MTAB-30773	P-MTAB-30774	P-MTAB-30775	P-MTAB-30776	P-MTAB-30777
Protocol Type	grow	nucleic acid extraction	labeling	hybridization	image_acquisition	bioassay_data_transformation
Protocol Description	Mef>mCD8GFP virgin females were crossed with UAS-lacZ, UAS-mblRNAi, UAS-600CTG or UAS-960CTG males and were maintained at 25C on standard medium in a 12:12 hour light-dark cycling humidified incubator. F1 third instar larvae were collected.	Total RNA was extracted from whole 3rd instar larvae using TRIzol reagent (Invitrogen). Three independent RNA isolations were performed for each of four genetic contexts (Mef>lacZ, Mef>mblRNAi, Mef>600CTG, Mef>960CTG). Validation of RNA quality was performed using both Nanodrop (OD260/OD280 *1.8) and Bioanalyser	Two-Color Microarray-Based Gene Expression Analysis-Low Input Quick Amp Labeling version 6.5, May 2010, Part Number G4140-90050	Agilent G2519F Drosophila Oligo Microarray (Design ID: 021791) format 4*44K	AgilentHD_GX_2Color on a Agilent C Scanner (scan resolution: 5mm, Tiff: 20 bit). Image analysis acoording to GE2_1010_Sep10 protocol.	Chip images are processed and normalized using the LOWESS method by Agilent software Feature Extraction. An in-house tool, Naomi (based on the quantile-quantile method), was used to re-normalize data.
SDRF File	E-MTAB-1469.sdrf.txt
Publication DOI	10.1093/hmg/ddt127