Comment[ArrayExpressAccession]	E-MTAB-1228				
Investigation Title	HS-30-48H Drosophila antennae				
Comment[Submitted Name]	HS-30-48H Drosophila antennae				
Experiment Description	Environmental temperature directly affects the concentration of chemicals in the gas phase. Therefore, if the olfactory system does not adapt to environmental conditions may provide inadequate information about distance to or direction of odor sources. Previous reports in Drosophila melanogaster show that temperature induces changes in olfactory sensitivity in complete individuals. Electrophysiological analysis demonstrates that these changes initiate at the olfactory receptor organs. In this experiment we try to identify particular genes responsible for olfactory adaptation to increasing temperatures using receptor organs of Drosophila after exposure to high temperatures. With this aim, wild-type Canton-S flies were subjected to 48-hour treatments at 30 degrees C. We use a direct approach selecting the specific tissue and making 'a priori' the gene election for the microarray analysis. Differential gene-expression analyses at the third antennal segments are performed comparing control and treated flies. 				
Experimental Design	stimulus_or_stress_design	in_vivo_design	co-expression_design		
Comment[AEExperimentType]	transcription profiling by array				
Comment[AEExperimentDisplayName]	Transcriptional profiling by array of drosophia antennae to identify genes responsible for olfactory adaptation to increasing environmental temperature				
Experimental Factor Name	temperature	environmental stress			
Experimental Factor Type	temperature	environmental stress			
Quality Control Type	biological_replicate				
Public Release Date	2013-08-01				
Person Last Name	Riveron Miranda				
Person First Name	Jacob				
Person Email	jriveron@liverpool.ac.uk				
Person Phone	(+0044)_7715290471				
Person Address					
Person Affiliation	Liverpool School of Tropical Medicine				
Person Roles	submitter				
Publication Status	In preparation				
Protocol Name	P-MTAB-27732	P-MTAB-27733	P-MTAB-27734	P-MTAB-27735	P-MTAB-27736
Protocol Type	grow	specified_biomaterial_action	nucleic_acid_extraction	labeling	bioassay_data_transformation
Protocol Description	The standard line of Drosophila melanogaster Canton-S, obtained from the Bloomington Stock Center (BSC, Indiana, USA) was used. Flies were bred at 21 +/1 degree C, with light/dark cycles of 12:12 hours, in 220 cc. bottles with a common yeast-sugar-agar medium.	 In the experimental group 2-8 days old flies were placed 48h at 30 +/- 1 degree C. For the control group, flies remained at growing temperature. Prior treatment experimental and control flies were transferred to new bottles with a near odorless medium composed by 5g/l agarose, 50g/l sucrose and water. With this measure we try to minimize the differential effect of environmental odor in the experimental group versus control. Third antennal segments were obtained by freezing flies in liquid nitrogen, breakage and collection. A high number of samples, approximately 4000 third antennal segments for each replicate, were collected to achieve a sufficient representation of genes with low expression levels that could be missing with other protocols. Each replicate contained 2 groups of 2000 segments collected from 11:00 am to 14:00 pm and 16:00 pm to 19:00 pm, respectively, to prevent misassignments of gene expression due to circadian fluctuations. 	Total RNA was purified with Nucleospin RNA II kit (Macherey-Nagel), following manufacturer's instructions. 	RNA processing, hybridization and scanning were performed at the Laboratory of Molecular Oncology, HUCA (Asturias, Spain) according the manufacturer's instructions, from 3ug total RNA for each of the 8 samples. 	Data were GC-RMA normalized with Bioconductor and the R project for statistical computing (Wu & Irizarry, 2004), using the BRB-Tools program developed at the Biometric Research Branch of the Division of Cancer Treatment & Diagnosis of the National Cancer Institute (Maryland, USA). 
Term Source Name	ArrayExpress				
Term Source File	www.ebi.ac.uk/arrayexpress				
SDRF File	E-MTAB-1228.sdrf.txt				
Publication Title	Transcriptional basis of the acclimation to high environmental temperature at the olfactory receptor organs of Drosophila melanogaster.				
Publication Author List	Riveron J, Boto T, Alcorta E				
PubMed ID	23590196				
Publication DOI	10.1186/1471-2164-14-259