Comment[ArrayExpressAccession]	E-MTAB-1011
Investigation Title	Tanscriptional profiling of heavy metal hyperaccumulator Sedum alfredii Hance (Crassulaceae) through RNA sequencing (RNA-seq) reveals molecular mechanisms of cadmium tolerance in plants
Comment[Submitted Name]	Tanscriptional profiling of heavy metal hyperaccumulator Sedum alfredii Hance (Crassulaceae) through RNA sequencing (RNA-seq) reveals molecular mechanisms of cadmium tolerance in plants
Experiment Description	RNA-seq was used to unveil the molelcualr mechanisms of Cd tolereance in Sedum alfredii Hance shoots
Experimental Design	treatment_comparison_design
Comment[AEExperimentType]	transcription profiling by high throughput sequencing
Experimental Factor Name	CellLine	Dose
Experimental Factor Type	cell_line	dose
Quality Control Type	biological_replicate 3 replicates
Public Release Date	2013-11-15
Person Last Name	Liu
Person First Name	Jian-Xiang
Person Mid Initials
Person Email	jianxiangliu@fudan.edu.cn
Person Phone	0086-21-65642163
Person Address	State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences,
Person Affiliation	Fudan University
Person Roles	submitter
PubMed ID	23755133
Publication Author List	Gao J, Sun L, Yang X, Liu JX
Publication Title	Transcriptomic analysis of cadmium stress response in the heavy metal hyperaccumulator Sedum alfredii Hance
Publication Status	submitted
Protocol Name	P-MTAB-25756	P-MTAB-25757	P-MTAB-25758	P-MTAB-25759	P-MTAB-25760
Protocol Description	Total RNA was extracted and quality checked with Agilent 2100 Bioanalyzer (Agilent, USA). mRNA was purified with Micropoly(A)PuristTM mRNA purification kit (Ambion, USA) following the DNase I digestion (Ambion, USA). cDNA was synthesized with GsuI-oligo dT primer and Superscript II reverse transcriptase (Invitrogen). cDNA library was constructed and sequenced with Illumina Solexa sequencing. After removing the low quality reads, the clean reads from Solexa sequencing in each sample were mapped to the assembled contigs and normalized to RPKM. 	S. alfredii Hance (HE) were obtained from an old Pb/Zn mine area in Zhejiang Province in China and cultured hydroponically in basal nutrient solution supplied with or without 100 uM CdCl2 for 8days.	no description provided	no description provided	no description provided
Protocol Type	sequencing	grow	specified_biomaterial_action	bioassay_data_transformation	nucleic_acid_extraction
Protocol Hardware	Illumina Genome Analyzer II
Comment[SecondaryAccession]	ERP001271
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/ERR102955-ERR102978
SDRF File	E-MTAB-1011.sdrf.txt
Publication DOI	10.1371/journal.pone.0064643