Investigation Title	Transcription profiling of brain in three adult humans and three adult chimpanzees
Comment[Submitted Name]	The DNA Sequence of Chimpanzee Chromosome 22 and Its Comparative Analysis with Human Ortholog, Chromosome 21
Experimental Design	species_design	transcription profiling by array
Experimental Design Term Source REF	mo	EFO
Comment[ArrayExpressReleaseDate]
Comment[AEMIAMESCORE]	4
Comment[ArrayExpressAccession]	E-MEXP-43
Comment[MAGETAB TimeStamp_Version]	2011-01-23 10:22:18 Last Changed Rev: 14857
Experimental Factor Name	organism
Experimental Factor Type	organism
Experimental Factor Term Source REF
Person Last Name	Cheong	Choi	Chen	Lee	Ren	Zhao	Wang	Fu	Zhang	Zhu	Reinhardt	Zheng	Hellmann	Pääbo	Nordsiek	Blöcker	Khaitovich	Sakaki	Yaspo	Wang	Park	Tsai	Saitou	Kohara	Kitano	Oota	Kim	Hsiao	Liu	Wu	Lehrach	BenKahla	Sudbrak	Kube	Galgoczy	Taenzer	Scharfe	Platzer	Watanabe	Hattori	Fujiyama	Toyoda	Taylor	Noguchi	Kuroki
Person First Name	J.-E.	S.-H.	Z.	Y.-S.	S.-X.	G.-P.	B.-F.	G.	X.-L.	G.-F.	R.	H.-J.	I.	S.	G.	H.	Philipp	Yoshiyuki	M.-L.	S.-Y.	H.-S.	S.-F.	N.	Y.	T.	S.	C.-G.	K.-J.	T.-T.	K.-M.	H.	A.	R.	M.	P.	S.	M.	M.	H.	M.	A.	A.	T.D.	H.	Y.
Person Mid Initials
Person Email																	khaitovich@eva.mpg.de
Person Phone																	 49 341 3550 520
Person Fax																	 49 341 3550 555
Person Address																	Deutscher Platz 6
Person Affiliation	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	Evolutionary Genetics	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization	unknown organization
Person Roles																	submitter
Person Roles Term Source REF																	mo
Quality Control Type
Quality Control Term Source REF
Replicate Type
Replicate Term Source REF
Normalization Type
Normalization Term Source REF
Date of Experiment
Public Release Date	2004-05-27
PubMed ID	15164055
Publication DOI	15164055
Publication Author List	H. Watanabe; A. Fujiyama; M. Hattori; T.D. Taylor; A. Toyoda; Y. Kuroki; H. Noguchi; A. BenKahla; H. Lehrach; M. Kube; R. Sudbrak; S. Taenzer; P. Galgoczy; M. Platzer; M. Scharfe; G. Nordsiek; H. Blöcker; I. Hellmann; P. Khaitovich; S. Pääbo; R. Reinhardt; H.-J. Zheng; X.-L. Zhang; G.-F. Zhu; B.-F. Wang; G. Fu; S.-X. Ren; G.-P. Zhao; Z. Chen; Y.-S. Lee; J.-E. Cheong; S.-H. Choi; K.-M. Wu; T.-T. Liu; K.-J. Hsiao; C.-G. Kim; S. Oota; T. Kitano; Y. Kohara; N. Saitou; S.-F. Tsai; H.-S. Park; S.-Y. Wang; M.-L. Yaspo; Yoshiyuki Sakaki
Publication Title	DNA sequence and comparative analysis of chimpanzee chromosome 22
Publication Status	journal_article
Publication Status Term Source REF	mo
Experiment Description	Gene expression profiling in brain of three adult humans and three adult chimpanzees
Protocol Name	P-MEXP-1595	P-MEXP-1591	P-MEXP-1592	Affymetrix:Protocol:Hybridization-EukGE-WS2v3[]	Affymetrix:Protocol:Percentile	Affymetrix:Protocol:ExpressionStat
Protocol Type	specified_biomaterial_action	nucleic_acid_extraction	labeling	hybridization	feature_extraction	bioassay_data_transformation
Protocol Description	all samples were dissected from deep frozen postmortem tissues without defreezing.	For each sample 200 mg of tissue was homogenized in 2 mL of TRIZol(R) solution at 4C for 3 minutes. Tolal RNA was isolated following the manifacturer instructions including the optional centrifugation step.  After isopropanol precipitation, samples were resuspended in 100 ul of formamide and purifyed using QIAGEN(R) RNeasy(R) protocol. RNA was elluted with 50 ul of water.	total RNA was labeled and purified following Affymetrix(R) protocol without any modifications, using ENZO(R) labeling kit.	Title: Fluidics Station Protocol. Description: 	Title: Affymetrix CEL Analysis (Percentile). Description: 	Title: Affymetrix CHP Analysis (ExpressionStat). Description:
Protocol Parameters		Extracted product;Amplification;	Amplification;Label used;Amount of nucleic acid labeled;
Protocol Hardware
Protocol Software				MicroArraySuite 5.0	MicroArraySuite 5.0	MicroArraySuite 5.0
Protocol Contact
Protocol Term Source REF
SDRF File	E-MEXP-43.sdrf.txt
Term Source Name	mo		ArrayExpress	mo	EFO
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php		http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/
Term Source Version