Comment[ArrayExpressAccession]	E-MEXP-3476					
Investigation Title	Alternative Reading Frame (ARF) overexpression in LN229 glioma cells					
Comment[Submitted Name]	Alternative Reading Frame (ARF) overexpression in LN229 glioma cells					
Comment[AEExperimentDisplayName]	Transcription profiling by array of alternative reading frame (ARF) overexpression in human LN229 glioma cells					
Comment[MIAMExpressLogin]	azerrouqi					
Comment[MIAMExpressSubmissionID]	7992					
Experiment Description	p14ARF inducible A5 glioma cells express p14ARF after cell exposure to 2 ug of doxycycline					
Experimental Design	in_vitro_design	co-expression_design	compound_treatment_design			
Comment[AEExperimentType]	transcription profiling by array					
Experimental Factor Name	COMPOUND	DOSE				
Experimental Factor Type	compound	dose				
Person Last Name	Zerrouqi					
Person First Name	Abdessamad					
Person Email	azerrou@emory.edu					
Person Phone	404-778-2267					
Person Affiliation	Winship Cancer Institute					
Person Address	Neurosurgery, 1365C Clifton Rd. N.E., Atlanta, Georgia, 30322, USA					
Person Roles	submitter					
Person Roles Term Source REF	MGED Ontology					
Quality Control Type	biological_replicate					
Public Release Date	2012-03-15					
Comment[ArrayExpressSubmissionDate]	2012-01-03 15:19					
Publication Title	Alternative Reading Frame (ARF) inhibits Tumor-Induced Angiogenesis by a Novel HDM2/SP1/TIMP3 signaling axis					
Publication Status	submitted					
Protocol Name	P-MTAB-24552	P-MTAB-24553	P-AFFY-2	P-AFFY-6	Affymetrix:Protocol:MAS4:ExpressionCall	Affymetrix:Protocol:Hybridization-Unknown
Protocol Description	A5 cells were cultured in DMEM with 2% Bovine serum and antibiotics. The expression of p14ARF was induced by treating cells with 2ug/ml of doxycycline. (Parameters: start time = 48, time unit = hours, min temperature = 37, temperature unit = C, media = DMEM)	Affymetrix has successfully isolated good quality total RNA from mammalian cells using QIAGEN's RNeasy Total RNA Isolation kit. (Parameters: Extracted product = total_RNA, Amplification = none)				
Protocol Type	grow	nucleic_acid_extraction	labeling	feature_extraction	bioassay_data_transformation	hybridization
Protocol Term Source REF	MGED Ontology	MGED Ontology				
Term Source Name	MGED Ontology	ArrayExpress				
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress				
SDRF File	E-MEXP-3476.sdrf.txt