Comment[ArrayExpressAccession]	E-MEXP-3409
Investigation Title	Transcriptional profiles of microRNAs from peripheral blood mononuclear cells of type 1 diabetes mellitus patients
Comment[Submitted Name]	Transcriptional profiles of microRNAs from peripheral blood mononuclear cells of type 1 diabetes mellitus patients
Comment[AEExperimentDisplayName]	Transcriptional profiles of microRNAs from peripheral blood mononuclear cells of type 1 diabetes mellitus patients
Comment[MIAMExpressLogin]	gpassos
Comment[MIAMExpressSubmissionID]	7830
Experiment Description	The microRNA oligo microarrays were used to determine expression profiles of peripheral blood mononuclear cells from type 1 diabetes mellitus (T1DM) patients, aiming the identification of possible disease related MicroRNAs.
Experimental Design	ex_vivo_design	co-expression_design	transcript_identification_design
Comment[AEExperimentType]	transcription profiling by array
Experimental Factor Name	Individual
Experimental Factor Type	individual
Person Last Name	Passos
Person First Name	Geraldo
Person Mid Initials	A
Person Email	passos@rge.fmrp.usp.br
Person Phone	+55 16 36 02 30 30
Person Fax	+55 16 36 33 00 69
Person Affiliation	University of Sao Paulo, Faculty of Medicine
Person Address	Genetics, Ave. Bandeirantes, 3900, Ribeir�o Preto, SP, 14040-900, Brazil
Person Roles	submitter
Person Roles Term Source REF	MGED Ontology
Quality Control Type
Public Release Date	2012-10-25
Comment[ArrayExpressSubmissionDate]	2011-10-25 18:58:57
Publication Status	not yet submitted
Protocol Name	P-MTAB-23995	P-MTAB-23996	P-AGIL-18	P-AGIL-19	P-AGIL-29
Protocol Description	Trizol reagent (Invitrogen) was used for total RNA extraction according to the manufacturer's instructions. The RNA samples were eluted in H2O+DEPC and stored in a -80ﾺC freezer. The yield was quantified by Nanodrop ND-1000 Spectrophotometer (Uniscience) and the integrity of RNA samples was assessed by microfluidic electrophoresis using Agilent RNA Nano 6000 chips and an Agilent 2100 Bioanalyzer.<br>(Parameters: Extracted product = total_RNA, Amplification = none)	Prior to RNA extraction the peripheral blood mononuclear cells from type 1 diabetes mellitus (T1DM) patients were isolated from whole blood using Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation.
Protocol Type	nucleic_acid_extraction	treatment	labeling	hybridization	image_acquisition
Protocol Term Source REF	MGED Ontology		MGED Ontology	MGED Ontology	MGED Ontology
Term Source Name	MGED Ontology	ArrayExpress
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress
SDRF File	E-MEXP-3409.sdrf.txt
PubMed ID	24279768
Publication Author List	Collares CV, Evangelista AF, Xavier DJ, Rassi DM, Arns T, Foss-Freitas MC, Foss MC, Puthier D, Sakamoto-Hojo ET, Passos GA, Donadi EA.
Publication Title	Identifying common and specific microRNAs expressed in peripheral blood mononuclear cell of type 1, type 2, and gestational diabetes mellitus patients.
Publication DOI	10.1186/1756-0500-6-491