Investigation Title	Identification of the epithelial progenitor niche for the Merkel cell lineage	
Comment[Submitted Name]	Identification of the epithelial progenitor niche for the Merkel cell lineage	
Experimental Design	cell_type_comparison_design	co-expression_design	all_pairs	transcription profiling by array	
Experimental Design Term Source REF	The MGED Ontology	The MGED Ontology		EFO	
Comment[SecondaryAccession]		
Comment[ArrayExpressReleaseDate]	2010-11-15	
Comment[AEMIAMESCORE]	5	
Comment[ArrayExpressAccession]	E-MEXP-2925	
Comment[MAGETAB TimeStamp_Version]	2010-09-30 18:51:00 Last Changed Rev: 13833	
Experimental Factor Name	CELL_TYPE	
Experimental Factor Type	cell_type	
Experimental Factor Term Source REF	
Person Last Name	Stumpfova	
Person First Name	Magda	
Person Mid Initials	
Person Email	ms2956@columbia.edu	
Person Phone	2128514545	
Person Fax	
Person Address	Dermatology, 1150 St. Nicholas Ave, RB312, New York, NY, 10032, USA	
Person Affiliation	Columbia University	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2010-11-15	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	Identification of the epithelial progenitor niche for the Merkel cell lineage	
Publication Status	
Publication Status Term Source REF	
Experiment Description	Transcriptional profile of murine touch dome keratinocytes sorted on the basis of alpha6-integrin, CD34, Sca1 and CD200 surface markers. Compared to the transcriptional profile of murine interfollicular epidermis (IFE) without the touch dome keratinocytes	
Protocol Name	P-MTAB-17171	P-AFFY-2	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6	Affymetrix:Protocol:MAS4:ExpressionCall	
Protocol Type	nucleic_acid_extraction	labeling	hybridization	feature_extraction	bioassay_data_transformation	
Protocol Description	Total RNA extracted from 200,000-1,000,000 sorted cells by Trizol method. 50ng of DNase1-treated RNA used for amplification and labeling using the GeneChip 3' IVT Express Kit. (Parameters: Extracted product = total_RNA, Amplification = RNA polymerases)					
Protocol Parameters	
Protocol Hardware	
Protocol Software			MicroArraySuite 5.0	MicroArraySuite 5.0	MicroArraySuite 4.0	
Protocol Contact	
Protocol Term Source REF	The MGED Ontology		mo		mo	
SDRF File	E-MEXP-2925.sdrf.txt	
Term Source Name	EFO	The MGED Ontology	NCI_thesaurus	ArrayExpress	The MGED Ontology	EFO	mo	
Term Source File	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	ncithesaurus.obo.alt	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version