Comment[ArrayExpressAccession]	E-MEXP-2294
Investigation Title	Transcription profiling of Arabidopsis thalianawild type and areb1 areb2 abf3 triple mutant seedlins after 6h of dehydration
Comment[Submitted Name]	areb1 areb2 abf3_dehydration 6h
Publication Title	AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation
Publication Author List	Takuya Yoshida, Yasunari Fujita, Hiroko Sayama, Satoshi Kidokoro, Kyonoshin Maruyama, Junya Mizoi, Kazuo Shinozaki and Kazuko Yamaguchi-Shinozaki
Publication DOI	10.1111/j.1365-313X.2009.04092.x
PubMed ID	19947981
Experimental Design	in_vivo_design	co-expression_design	stimulus_or_stress_design	transcription profiling by array
Experimental Design Term Source REF		The MGED Ontology	The MGED Ontology	EFO
Comment[MIAMExpressLogin]	jircasarray																							
Comment[MIAMExpressSubmissionID]	5930																							
Experiment Description	Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates with stress treatment of dehydration for 6 h.																							
Experimental Factor Name	GENOTYPE																							
Experimental Factor Type	genotype																							
Person Last Name	Maruyama																							
Person First Name	Kyonoshin																							
Person Email	kyonosin@jircas.affrc.go.jp																							
Person Phone	81 298 38 6642																							
Person Affiliation	JIRCAS																							
Person Address	Biological Resources, 1-1,Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan																							
Person Roles	submitter																							
Person Roles Term Source REF	MGED Ontology																							
Quality Control Type																								
Public Release Date	2010-08-04																							
Comment[ArrayExpressSubmissionDate]	2009-08-05																							
Protocol Name	P-MTAB-5028	P-MTAB-5029	P-MTAB-5030	P-MTAB-5031	P-MTAB-5032	P-MTAB-5033	P-MTAB-5034
Protocol Description	The labeling experiment was performed according to the manufacture's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1).<br>(Parameters: Amplification = none, Mass unit = Micro gram)	Plants were transferred from GM agar plates on 3MM-papers and then were dehydrated for 6 h. After the dehydration treatment, the plants were harvested, immediately frozen by liquid nitrogen and stored at a deep freezer.	The scanning was performed according to the manufactureﾒs protocol (Agilent technologies, A Method for Quantifying the performance of a DNA Microarray Scanner, Publication Number 5988-9498EN).<br>(Parameters: Scanning hardware = DNA Microarray Scanner BA [Agilent Technologies], Scanning software = Feature Extraction Software [Agilent])	Total RNAs were isolated using the RNAiso Reagent (Takara, Japan).<br>(Parameters: Extracted product = total_RNA, Amplification = none)	The labeling experiment was performed according to the manufacture's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1).<br>(Parameters: Amplification = none, Mass unit = Micro gram)	The growth conditions of the plants are as follows: The plants were grown in the plastic dishes (50 plants/plastic dish) containing germination medium (GM). GM contained Murashige and Skoog salts, 1% sucrose (WAKO, Osaka, Japan) and 0.83% Bacto-agar (Difco, Detroit, MI, USA). The plants were grown for 2 weeks in a growth chamber at 22C and 50-60% humidity under 16 hr light/8 hr dark.<br>(Parameters: time unit = seconds, temperature unit = C)	The hybridization experiment was performed according to the manufacture's protocol (Agilent technologies, Low RNA Input Fluorescent Linear Amplification Kit Protocol, Version 5.0.1).<br>(Parameters: Chamber type = OTHER: Robbins Scientific 1040-60-1AG, Quantity of label target used = 1, Mass unit = Micro gram, Tiny time unit = seconds, Volume unit = Nano litre, temperature = 65)																	
Protocol Type	labeling	specified_biomaterial_action	image_acquisition	nucleic_acid_extraction	labeling	grow	hybridization																	
Protocol Term Source REF	MGED Ontology	MGED Ontology	MGED Ontology	MGED Ontology	MGED Ontology	MGED Ontology	MGED Ontology																	
Term Source Name	MGED Ontology		The MGED Ontology	EFO
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php																							
SDRF File	E-MEXP-2294.sdrf.txt