Investigation Title	Transcription profiling of muscular and neuronal mouse mutant: spinal muscular atrophy(SMA) 	
Comment[Submitted Name]	SMA muscular and neuronal	
Experimental Design	disease_state_design	transcription profiling by array	
Experimental Design Term Source REF	mo	EFO	
Comment[ArrayExpressReleaseDate]	2005-05-20	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressAccession]	E-MEXP-131	
Comment[MAGETAB TimeStamp_Version]	2010-08-09 21:03:53 Last Changed Rev: 13058	
Experimental Factor Name	Organism Part	Genotype	Disease State	
Experimental Factor Type	organism_part	individual_genetic_characteristic	disease_state	
Experimental Factor Term Source REF	
Person Last Name	Melki	Courageot	Olaso	Joshi	Roblot	Fernandez	
Person First Name	Judith	Sabrina	Robert	Vandana	Natacha	Julien	
Person Mid Initials	
Person Email			olaso@cng.fr	
Person Phone			01 60 87 45 53	
Person Fax			07 60 87 45 50	
Person Address			2 Rue Gaston Cremieux	
Person Affiliation	EMI223	EMI223	EMI223	EMI223	EMI223	EMI223	
Person Roles			submitter	
Person Roles Term Source REF			mo	
Quality Control Type	biological_replicate	
Quality Control Term Source REF	The MGED Ontology	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2005-05-20	
PubMed ID	16118268	
Publication DOI	16118268	
Publication Author List	Olaso, Robert; Joshi, Vandana; Fernandez, Julien; Roblot, Natacha; Courageot, Sabrina; Bonnefont, Jean Paul; Melki, Judith	
Publication Title	Activation of RNA metabolism-related genes in mouse but not human tissues deficient in SMN	
Publication Status	journal_article	
Publication Status Term Source REF	mo	
Experiment Description	Study of gene expression profiles of muscular and neuronal mouse mutant of spinal muscular atrophy(SMA). Pre and post symptomatic stage disease have been analyzed.	
Protocol Name	P-MEXP-4186	P-MEXP-4141	P-MEXP-4144	P-MEXP-4160	
Protocol Type	specified_biomaterial_action	nucleic_acid_extraction	labeling	hybridization	
Protocol Description	Muscular (HSA-Cre, Smn&#916;7/F7) or neuronal (NSE-Cre, Smn&#916;7/F7) mutant mice were generated as previously described (Frugier, HMG 2000, Diaz JCB 2001). Mice were maintained on C57BL/6J genetic background. (Smn+/F7) animals from litter were used as controls. Animals were genotyped by PCR amplification of DNA extracted from tail biopsies (Miniou 1999, Frugier 2000, Gurney et al., 1994). All animal procedures were performed in accordance with institutional guidelines (agreement A91-228-2 and 3429).	RNA extraction<br> Tissues were dissected and stored at -80C. Total RNA was extracted using Trizol reagent (Invitrogen) according <br> to the manufacturer's instruction and purified using RNeasy columns (Quiagen). <br> RNA concentration and integrity were assessed according to absorbance at 260 and 280 nm and gel electrophoresis<br> 	Briefly, labelled cDNA was synthesized from 20 ug of total RNA from control or mutant tissus and <br> labelled with the indicated dye (Cy3 or Cy5, Perkin Elmer) using direct labelling cDNA synthesis kit (Agilent G2557A).	Cy5 and Cy3 labelled cDNA were mixed with cot1 DNA (Invitrogen) and deposition control target (Operon), <br> then the hybridisation mixture was pipette onto the slide, covered with a coverslip. The slide was placed into<br> a hybridisation chamber (Scienion AG, Germany), which was submerged in a 65C waterbath for 17 hours.	
Protocol Parameters		Amplification;Extracted product;	Label used;Amount of nucleic acid labeled;Amplification;	temperature;Volume;time;Quantity of label target used;Chamber type;	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF	
SDRF File	E-MEXP-131.sdrf.txt	
Term Source Name	mo		NCI_thesaurus	mgd	The MGED Ontology	ArrayExpress	The MGED Ontology	mo	EFO	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php		ncithesaurus.obo.alt	http://www.informatics.jax.org/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	
Term Source Version