Investigation Title Transcription profiling of mouse IL-12+IL-18 treated and Yersinia enterocolitica infected C57BL/6 NK cells Comment[Submitted Name] B6_IL1218_WAP Experimental Design unknown_experiment_design_type transcription profiling by array Experimental Design Term Source REF EFO Comment[SecondaryAccession] GSE9735 Comment[ArrayExpressReleaseDate] 2009-01-13 Comment[AEMIAMESCORE] 4 Comment[ArrayExpressAccession] E-GEOD-9735 Comment[MAGETAB TimeStamp_Version] 2010-08-09 15:40:14 Last Changed Rev: 13058 Experimental Factor Name Experimental Factor Type Experimental Factor Term Source REF Person Last Name Hoffmann Person First Name Reinhard Person Mid Initials Person Email reinhard.hoffmann@lrz.tum.de Person Phone Person Fax Person Address Institute for medical microbiology, Trogerstr. 30, Munich, 81675, Germany Person Affiliation Institute for medical microbiology Person Roles submitter Person Roles Term Source REF The MGED Ontology Quality Control Type Quality Control Term Source REF Replicate Type Replicate Term Source REF Normalization Type Normalization Term Source REF Date of Experiment Public Release Date 2009-01-13 PubMed ID Publication DOI Publication Author List Publication Title Publication Status Publication Status Term Source REF Experiment Description Dataset of IL-12+IL-18 trated and Yersinia enterocolitica infected C57BL/6 NK cells Experiment Overall Design: NK cells were isolated from mouse spleen, grown on IL-2 in vitro, stimlated wih Y. enterocolitica WA(pYV) or left uninfected. Experiment Overall Design: 3 conditions, 3 biological replicates each Protocol Name P-G9735-2 P-G9735-1 P-AFFY-6 Protocol Type nucleic_acid_extraction labeling feature_extraction Protocol Description Qiagen Rneasy Micro kit according to manufacturer's recommendations Ambion MessageAmp kit acording to manufacturers recommendations Title: Affymetrix CEL analysis. Description: Protocol Parameters Protocol Hardware Protocol Software MicroArraySuite 5.0 Protocol Contact Protocol Term Source REF SDRF File E-GEOD-9735.sdrf.txt Term Source Name NCBI Taxonomy The MGED Ontology ArrayExpress EFO The MGED Ontology Term Source File http://www.ncbi.nlm.nih.gov/Taxonomy/ http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/arrayexpress http://www.ebi.ac.uk/efo/ http://mged.sourceforge.net/ontologies/MGEDontology.php Term Source Version