Investigation Title	Transcription profiling of human embryonic stem cells, early stage embryoid bodies, and hES-derived blast cells.	
Comment[Submitted Name]	Expression data from human embryonic stem cells, early stage embryoid bodies, and hES-derived blast cells.	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[AEMIAMESCORE]	4	
Comment[ArrayExpressReleaseDate]	2008-06-16	
Comment[SecondaryAccession]	GSE8884	
Comment[ArrayExpressAccession]	E-GEOD-8884	
Comment[MAGETAB TimeStamp_Version]	2010-08-09 11:30:49 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Hipp	
Person First Name	Jennifer	
Person Mid Initials	A.	
Person Email	jenhipp@wfubmc.edu	
Person Phone	
Person Fax	
Person Address	Atala, Institute for Regenerative Medicine, Wake Forest School of Medicine, Medical Center Blvd, Winston-Salem, 27157, USA	
Person Affiliation	Wake Forest School of Medicine	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2008-06-16	
PubMed ID	17999768	
Publication DOI	17999768	
Publication Author List	Shi-Jiang Lu, Jennifer A Hipp, Qiang Feng, Jason D Hipp, Robert Lanza, Anthony Atala	
Publication Title	GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	To understand the differentiation process of embryonic stem cells into hemangioblasts, gene expression profiles of ES, EB and Blast cells (BL) were analyzed. Experiment Overall Design: Total RNA was isolated from purified BC cells, day3.5-EBs and undifferentiated ESCs (from two hESC lines, H1 and H9) using the Qiagen RNAeasy kit  We sought to obtain homogeneous populations of cells at each developmental stage in order to increase the purity of each sample.	
Protocol Name	P-G8884-2	P-G8884-1	P-G8884-4	P-G8884-3	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6	
Protocol Type	specified_biomaterial_action	grow	nucleic_acid_extraction	labeling	hybridization	feature_extraction	
Protocol Description	see supplementary information Lu et.al, Nature Methods, 2007 Jun;4(6):501-9.	see supplementary information Lu et.al, Nature Methods, 2007 Jun;4(6):501-9.	Total RNA was extracted by Qiagen RNAeasy kiit and was performed according to the manufacturer's instructions.	Biotinylated cRNA were prepared according to the standard Affymetrix protocol	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software					MicroArraySuite 5.0	MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF					mo	
SDRF File	E-GEOD-8884.sdrf.txt	
Term Source Name	mo		The MGED Ontology	ArrayExpress	EFO	The MGED Ontology	mo	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php		http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version