Investigation Title Transcription profiling of mouse splenic and small intestine lamina propria macrophages Comment[Submitted Name] Comparison of splenic and small intestine lamina propria macrophages Experimental Design unknown_experiment_design_type transcription profiling by array Experimental Design Term Source REF EFO Comment[AEMIAMESCORE] 3 Comment[ArrayExpressReleaseDate] 2008-06-16 Comment[SecondaryAccession] GSE8868 Comment[ArrayExpressAccession] E-GEOD-8868 Comment[MAGETAB TimeStamp_Version] 2010-08-09 11:22:44 Last Changed Rev: 13058 Experimental Factor Name Experimental Factor Type Experimental Factor Term Source REF Person Last Name Denning Person First Name Tim Person Mid Initials Person Email Person Phone Person Fax Person Address La Jolla Institute for Allergy and Immunology, 9420 Athena Circle, La Jolla, 92037, USA Person Affiliation La Jolla Institute for Allergy and Immunology Person Roles submitter Person Roles Term Source REF The MGED Ontology Quality Control Type Quality Control Term Source REF Replicate Type Replicate Term Source REF Normalization Type Normalization Term Source REF Date of Experiment Public Release Date 2008-06-16 PubMed ID 17873879 Publication DOI 17873879 Publication Author List Timothy L Denning, Yi-chong Wang, Seema R Patel, Ifor R Williams, Bali Pulendran Publication Title Lamina propria macrophages and dendritic cells differentially induce regulatory and interleukin 17-producing T cell responses. Publication Status journal_article Publication Status Term Source REF The MGED Ontology Experiment Description The intestinal immune system must elicit robust immunity against harmful pathogens but restrain immune responses directed against commensal microbes and dietary antigens. The mechanisms that maintain this dichotomy are poorly understood. Here we describe a population of CD11b+F4/80+CD11c– macrophages in the lamina propria (LP) that express several anti-inflammatory molecules including interleukin 10 (IL-10), but little or no pro-inflammatory cytokines, even upon stimulation with Toll-like receptor (TLR) ligands. These macrophages induced, in a manner dependent on IL-10, retinoic acid and exogenous transforming growth factor-β, differentiation of FoxP3+ regulatory T cells. In contrast, LP CD11b+ dendritic cells elicited IL-17 production. This IL-17 production was suppressed by LP macrophages, indicating that a dynamic interplay between these subsets may influence the balance between immune activation and tolerance. Splenic or small intestine lamina propria CD11b+11c- cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. We sought to determine the unique genetic profile of small intestine lamina propria CD11b+11c- cells. Experiment Overall Design: 4 samples analyzed, 2 spleen and 2 intestine Protocol Name P-G8868-2 P-G8868-1 P-G8868-4 P-G8868-3 Affymetrix:Protocol:Hybridization-Unknown P-AFFY-6 Affymetrix:Protocol:ExpressionStat Protocol Type specified_biomaterial_action grow nucleic_acid_extraction labeling hybridization feature_extraction bioassay_data_transformation Protocol Description No treatment involved Freshly isolated, then FACS-sorted for CD11b+11c- cells Qiagen Rneasy Mini Kit extraction of total RNA was performed according to the manufacturer's instructions. As per Vanderbilt Microarray Shared Resource Standard Protocol Title: Affymetrix Generic Hybridization. Description: Title: Affymetrix CEL analysis. Description: Title: Affymetrix CHP Analysis (ExpressionStat). Description: Protocol Parameters Protocol Hardware Protocol Software MicroArraySuite 5.0 MicroArraySuite 5.0 MicroArraySuite 5.0 Protocol Contact Protocol Term Source REF mo The MGED Ontology SDRF File E-GEOD-8868.sdrf.txt Term Source Name The MGED Ontology ArrayExpress EFO The MGED Ontology mo Term Source File http://mged.sourceforge.net/ontologies/MGEDontology.php http://www.ebi.ac.uk/arrayexpress http://www.ebi.ac.uk/efo/ http://mged.sourceforge.net/ontologies/MGEDontology.php http://mged.sourceforge.net/ontologies/MGEDontology.php Term Source Version