Investigation Title	Transcription profiling of human skeletal muscle from 55-80 year old individuals fed low and high protein diets	
Comment[Submitted Name]	Inadequate protein intake affects skeletal muscle transcript profiles in older humans	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[ArrayExpressReleaseDate]	2008-06-16	
Comment[SecondaryAccession]	GSE8441	
Comment[AEMIAMESCORE]	3	
Comment[ArrayExpressAccession]	E-GEOD-8441	
Comment[MAGETAB TimeStamp_Version]	2010-08-09 10:41:38 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Campbell	
Person First Name	Wayne	
Person Mid Initials	
Person Email	campbellw@purdue.edu	
Person Phone	
Person Fax	
Person Address	Foods and Nutrition, Purdue University, 700 West State Street , West Lafayette, 47907, USA	
Person Affiliation	Purdue University	
Person Roles	submitter	
Person Roles Term Source REF	The MGED Ontology	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2008-06-16	
PubMed ID	17490972	
Publication DOI	17490972	
Publication Author List	Anna E Thalacker-Mercer, James C Fleet, Bruce A Craig, Nadine S Carnell, Wayne W Campbell	
Publication Title	Inadequate protein intake affects skeletal muscle transcript profiles in older humans.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	Inadequate dietary protein intake causes adverse changes in the morphology and function of skeletal muscle.  These changes may be reflected in early alterations in muscle mRNA levels. Microarray analysis was used to assess whether inadequate protein intake differentially affects skeletal muscle transcript levels and expression profiles in older adults. Experiment Overall Design: 11 older males and females (aged 55-80 y) consumed controlled diets that contained 1.2 g protein•kg-1•d-1 (adequate protein) for one wk and then  0.5 g protein•kg-1•d-1 (inadequate protein; IP group; n=11) for a second wk.  RNA was isolated from fasting-state vastus lateralis biopsies obtained at the end of each period and the transcript levels were determined using the Affymetrix U133A array	
Protocol Name	P-G8441-2	P-G8441-1	P-G8441-4	P-G8441-3	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6	
Protocol Type	specified_biomaterial_action	grow	nucleic_acid_extraction	labeling	hybridization	feature_extraction	
Protocol Description	controlled diets that contained 1.2 g protein•kg-1•d-1 (adequate protein) for one wk followed by 0.5 g protein•kg-1•d-1 (inadequate protein) for a second wk.  Fasting state vastus lateralis muscle biopsies were taken on day 7 and day 14 of both dietary trials	NA	Total RNA was isolated from the frozen muscle sample using Tri-Reagent following the manufacturer’s instructions.  Isolated total RNA was further purified using RNeasy Mini Kits following manufacturer’s protocol	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from a minimum of 5 ug total RNA	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software					MicroArraySuite 5.0	MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF					mo	
SDRF File	E-GEOD-8441.sdrf.txt	
Term Source Name	mo		The MGED Ontology	ArrayExpress	EFO	The MGED Ontology	mo	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php		http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version