Investigation Title	Transcription profiling of human prostate adenocarcinoma cells	
Comment[Submitted Name]	CCFAlmasan_CaP1	
Experimental Design	time_series_design	dose_response_design	cell_type_comparison_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF	mo		mo	mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-09-25	
Comment[SecondaryAccession]	GSE762	
Comment[AEMIAMESCORE]	4	
Comment[SecondaryAccession]	GDS723	
Comment[SecondaryAccession]	GDS722	
Comment[SecondaryAccession]	GDS721	
Comment[SecondaryAccession]	GDS720	
Comment[SecondaryAccession]	GDS719	
Comment[ArrayExpressAccession]	E-GEOD-762	
Comment[MAGETAB TimeStamp_Version]	2011-01-23 01:42:30 Last Changed Rev: 14857	
Experimental Factor Name	Time	Dose	CellLine	
Experimental Factor Type	time	dose	cell_line	
Experimental Factor Term Source REF	
Person Last Name	Hissong	
Person First Name	John Gilmary	
Person Mid Initials	
Person Email	hissonj@ccf.org	
Person Phone	
Person Fax	
Person Address	Almasan Lab NB40, Cancer Biology, Lerner Research Institute Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, 44195, USA	
Person Affiliation	Lerner Research Institute Cleveland Clinic Foundation	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-09-25	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	
Publication Status	
Publication Status Term Source REF	
Experiment Description	The experiment design was multifold, consisting of a time course experiment on LNCaP C4-2 human prostate adenocarcinoma cells following irradiation to a dose of 10 Sv from a Cesium-137 mixed gamma and beta source, with two additional samples, one exposed to radiation in four fractions instead of all at once, and one containing the parent LNCaP cell line.  PolyA+ RNA was extracted, processed and hybridized to the Affymetrix (Santa Clara, CA, www.affymetrix.com) HGU-95 (Av1-E) chip sets, and then washed, stained and scanned according to Affymetrix's protocols contained in the GeneChip(R) Expression Analysis Manual.  Transcript abundance data from the scans was processed initially with Affymetrix' Microarray Suite 5(R), then by Silicon Genetics' (Redwood City, CA, www.silicongenetics.com) GeneSpring(R).	
Protocol Name	
Protocol Type	
Protocol Description	
Protocol Parameters	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF	
SDRF File	E-GEOD-762.sdrf.txt	
Term Source Name	ncbitax	The MGED Ontology	ArrayExpress	mo	EFO	
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	
Term Source Version