Comment[ArrayExpressAccession]	E-GEOD-66316
MAGE-TAB Version	1.1						
Public Release Date	2016-02-01
Investigation Title	Expression data from adult male Sprague Dawley rat liver, investigating how pomegranate skin extract prevents non alcoholic fatty liver diseases						
Comment[Submitted Name]	Expression data from adult male Sprague Dawley rat liver, investigating how pomegranate skin extract prevents non alcoholic fatty liver diseases
Experiment Description	Pomegranate skin extract could prevent fatty liver due to high fat diet in adult male Sprague Dawley rat. There are 3 groups of rats, feeding chow diet, high fat diet and high fat diet combined with pomegrante skin extract. After 8 weeks feeding, high fat diet group developped fatty liver but the other two groups still have healthy liver. We used microarrays to detail the global programme of gene expression underlying the fatty liver development and preventive effect of pomegranate skin extract on fatty liver. There are 3 treatment groups and 6 replicates for each group. The 3 treatment groups are control group feeding chow diet, high fat group feeding high fat diet and PE group feeding high fat diet and pomegranate skin extract. Rat livers were collecteded for RNA extraction and hybridization on Affymetrix microarrays.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	shi	Shi					
Person First Name	yujie	Yujie					
Person Email	yujie.shi@rd.nestle.com						
Person Affiliation	Nestle research center- Beijing hub						
Person Phone	+8613426001743						
Person Address	Nestle research center- Beijing hub, No.5 building No.5 Road Dijin, Beijing, China						
Person Roles	submitter						
Protocol Name	P-GSE66316-1	P-GSE66316-5	P-GSE66316-6	P-GSE66316-2	P-GSE66316-3	P-GSE66316-4	P-GSE66316-7
Protocol Description	The data were analyzed with Expression console software using Robust Multichip Average(RMA) as normalization method. ID_REF =  VALUE = RMA signal intensity	Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45˚C on Affymetrix Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.	Then rats were fasted overnight and sacrificed. Liver samples were collected after sacrifice and immediately stored in liquid nitrogen tank until RNA extraction.	Specific pathogen free Sprague Dawley (SD) male rats were housed in a temperature (22˚C-28˚C) and humidity (60%)-controlled animal room and maintained on a 12-h light/12-h dark cycle (light from 08:00 a.m. to 08:00 p.m.) ad libitum to food and water for 8 weeks.	The extraction of total RNA was performed using Agencourt RNAdvance tissue kit according to the manufacturer's instructions.	GeneChips were scanned using the GeneArray Scanner G3000 7G system.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	tissue condition	diet group					
Experimental Factor Type	tissue condition	diet group					
Comment[SecondaryAccession]	GSE66316						
Comment[GEOReleaseDate]	2016-02-01						
Comment[ArrayExpressSubmissionDate]	2015-02-26						
Comment[GEOLastUpdateDate]	2016-02-01						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-66316.sdrf.txt