Investigation Title	Transcription profiling of mouse mammary gland from 	
Comment[Submitted Name]	Expression data from mammary glands of transgenic mice	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[ArrayExpressReleaseDate]	2008-06-14	
Comment[SecondaryAccession]	GSE6581	
Comment[AEMIAMESCORE]	3	
Comment[ArrayExpressAccession]	E-GEOD-6581	
Comment[MAGETAB TimeStamp_Version]	2010-08-06 21:14:18 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Klein	
Person First Name	Andreas	
Person Mid Initials	
Person Email	andreas.klein@charite.de	
Person Phone	
Person Fax	
Person Address	Charite, Arnimallee 22, Berlin, 14195, Germany	
Person Affiliation	
Person Roles	
Person Roles Term Source REF	
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2008-06-14	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	
Publication Status	
Publication Status Term Source REF	
Experiment Description	The aim of our work was the comparison of human and mouse gene expression data and to identify a conserved breast tumor gene set. The results encourage the usefulness of transgenic mice as a model for human breast cancer formation and therapy. Experiment Overall Design: The aim of our work was to establish a database for breast cancer gene expression data in order to compare human and mouse breast cancer. We identified human and mouse homologues genes and compared the expression profile of 24 human breast tumors with six WAP-SVT/t breast tumors (WAP-SVT/t animals, line 8). Our studies confirmed the heterogeneity in gene expression of human as well as mouse breast cancer cells. However, 63 genes were found to be differentially expressed (upregulated: 40; downregulated: 23 genes) in at least 75% of the breast tumors of both species.	
Protocol Name	P-G6581-2	P-G6581-1	P-G6581-4	P-G6581-3	Affymetrix:Protocol:Hybridization-Unknown	P-AFFY-6	
Protocol Type	specified_biomaterial_action	grow	nucleic_acid_extraction	labeling	hybridization	feature_extraction	
Protocol Description	breast cancer	breast cancer	RNAzol extraction of total RNA was performed according to the manufacturer's instructions.	The doublestranded DNA was transcribed in vitro into biotinylated cRNA	Title: Affymetrix Generic Hybridization. Description: 	Title: Affymetrix CEL analysis. Description: 	
Protocol Parameters	
Protocol Hardware	
Protocol Software					MicroArraySuite 5.0	MicroArraySuite 5.0	
Protocol Contact	
Protocol Term Source REF					mo	
SDRF File	E-GEOD-6581.sdrf.txt	
Term Source Name	The MGED Ontology		ArrayExpress	EFO	mo	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php		http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version