Comment[ArrayExpressAccession]	E-GEOD-64554
MAGE-TAB Version	1.1				
Public Release Date	2015-11-22
Investigation Title	GE/miRNA expression profile of Human Epicardial Adipose Tissue (EAT) and Subcutaneous Adipose Tissue (SAT) in Patients with Coronary Artery Disease (CAD) vs. Controls (CTRL) - PART 1 - Genes				
Comment[Submitted Name]	GE/miRNA expression profile of Human Epicardial Adipose Tissue (EAT) and Subcutaneous Adipose Tissue (SAT) in Patients with Coronary Artery Disease (CAD) vs. Controls (CTRL) - PART 1 - Genes
Experiment Description	Gene expression profiles of Human EAT vs. SAT (CTRL & CAD). The aim of the present study was to assess a gene expression chart characterizing EAT vs. SAT, and CAD vs. CTRL. Results provide the information that EAT is characterized by a differential expression of different genes when compared to its reference tissue (SAT), and that EAT is characterized by specific gene expression changes in patients with CAD. RNA obtained from EAT & SAT of the same patients (paired samples). Comparisons: EAT vs. SAT (paired samples) & CAD vs. CTRL				
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl			
Person Last Name	Moschetta	Vacca	D'Orazio		
Person First Name	Antonio	Michele	Andria		
Person Email	geo@ncbi.nlm.nih.gov				
Person Affiliation	IRCCS Ospedale Oncologico di Bari				
Person Address	IRCCS Ospedale Oncologico di Bari, Via Orazio Flacco, 65, Bari, Italy				
Person Roles	submitter				
Protocol Name	P-GSE64554-1	P-GSE64554-3	P-GSE64554-4	P-GSE64554-2	P-GSE64554-5
Protocol Description	The data were normalised using quantile normalization (background was not subtracted) with Illumina Genome Studio Software; items with detection p values >0.001 or AVG Signal <100 in more than 1 group were excluded. Data were analyzed with non parametric analysis plus False Discovery Rate, FDR. ID_REF =  VALUE = Quantile Normalized AVG signal Detection Pval = 	Standard Illumina Protocol	Standard Illumina hybridization protocol	Total RNA was extracted using the Qiagen miRNeay Kit, following manufacturer's instructions. Quality control was performed with Biorad Experion.	Standard Illumina scanning protocol
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	disease state	organism part	age		
Experimental Factor Type	disease state	organism part	age		
Comment[SecondaryAccession]	GSE64554				
Comment[GEOReleaseDate]	2015-11-22				
Comment[ArrayExpressSubmissionDate]	2014-12-29				
Comment[GEOLastUpdateDate]	2015-11-22				
Comment[AEExperimentType]	transcription profiling by array				
Comment[AdditionalFile:Data1]	GSE64554_non-normalized.txt				
SDRF File	E-GEOD-64554.sdrf.txt