Comment[ArrayExpressAccession]	E-GEOD-58754
MAGE-TAB Version	1.1				
Public Release Date	2014-06-25
Investigation Title	Expression data from Wild type and Wip1 knockout Hematopoietic stem cell				
Comment[Submitted Name]	Expression data from Wild type and Wip1 knockout Hematopoietic stem cell
Experiment Description	Hematopoietic stem cell (HSC) aging underlies many age-related hematopoietic disorders. Accumulation of DNA damage is a hallmark of HSC aging. Wild-type p53-induced phosphatase 1 (Wip1) is a homeostatic regulator of DNA damage response. We used microarrays to detail the global programme of gene expression in Wip1 KO HSC Wild-type p53-induced phosphatase 1 (Wip1) knockout HSC and Wild type HSC were selected  for RNA extraction and hybridization on Affymetrix microarrays.				
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl			
Person Last Name	Wang	Ju	Yi		
Person First Name	Hu	Zhenyu	Weiwei		
Person Email	wanghu19860315@163.com				
Person Affiliation	hangzhou normal university				
Person Address	hangzhou normal university, wenyixi road 1378, hangzhou, China				
Person Roles	submitter				
Protocol Name	P-GSE58754-1	P-GSE58754-3	P-GSE58754-4	P-GSE58754-2	P-GSE58754-5
Protocol Description	The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. ID_REF =  VALUE = MAS5.0 signal intensity	Cy3-cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).	Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.	Trizol extraction of total RNA was performed according to the manufacturer's instructions.	GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	PHENOTYPE				
Experimental Factor Type	phenotype				
Comment[SecondaryAccession]	GSE58754				
Comment[GEOReleaseDate]	2014-06-25				
Comment[ArrayExpressSubmissionDate]	2014-06-24				
Comment[GEOLastUpdateDate]	2014-06-26				
Comment[AEExperimentType]	transcription profiling by array				
SDRF File	E-GEOD-58754.sdrf.txt