Comment[ArrayExpressAccession]	E-GEOD-58193
MAGE-TAB Version	1.1						
Public Release Date	2014-06-04
Investigation Title	Characterizing the profiles of Ebf1 in mouse fat cells.						
Comment[Submitted Name]	Characterizing the profiles of Ebf1 in mouse fat cells.
Experiment Description	gene expression data from mouse adipocyte, with and without Ebf1 knock-down To ascertain the functional targets of Ebf1 in adipocytes, we depleted Ebf1 from mature 3T3-L1 cells by transducing them with lentivirus harboring one of two different Ebf1-specific hairpins or a scrambled control. We then performed expression profiling using Affymetrix arrays with triplicate samples for each hairpin.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Rosen	Griffin	Zhou	Kang	Zhang	Mikkelsen	Rosen
Person First Name	Evan	Michael	Yiming	Sona	Xiaolan	Tarjei	Evan
Person Mid Initials		J				S	D
Person Email	erosen@bidmc.harvard.edu						
Person Affiliation	Beth Israel Deconess Medical Center						
Person Address	Endocrinology, Beth Israel Deconess Medical Center, 3 Blackfan Cir, Boston, MA, USA						
Person Roles	submitter						
Protocol Name	P-GSE58193-1	P-GSE58193-4	P-GSE58193-5	P-GSE58193-2	P-GSE58193-3	P-GSE58193-6	
Protocol Description	RMA procedure in BioConductor package for summarization and normalization ID_REF =  VALUE = RMA normalized signals	labeling with Encore Biotin Module.	As manufacturer's instruction	Mature 3T3-L1 cells were subjected to 3-4 serial infections with virus, and selected with 5 μg/ml puromycin for at least 8 days prior to harvest.	amplification with the NuGEN Ovation WTA Pico kit	As manufacturer's instruction	
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	nucleic acid extraction protocol	array scanning protocol	
Experimental Factor Name	LENTIVIRUS						
Experimental Factor Type	lentivirus						
Comment[SecondaryAccession]	GSE58193						
Comment[GEOReleaseDate]	2014-06-04						
Comment[ArrayExpressSubmissionDate]	2014-06-03						
Comment[GEOLastUpdateDate]	2014-06-04						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-58193.sdrf.txt