Investigation Title	Transcription profiling of mouse pre-metastatic lungs	
Comment[Submitted Name]	Gene expression analysis of pre-metastatic lungs	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[SecondaryAccession]	GSE5778	
Comment[ArrayExpressReleaseDate]	2009-01-13	
Comment[AEMIAMESCORE]	3	
Comment[ArrayExpressAccession]	E-GEOD-5778	
Comment[MAGETAB TimeStamp_Version]	2010-08-06 17:31:49 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Watanabe	
Person First Name	Akira	
Person Mid Initials	
Person Email	watanabe-a@lsbm.org	
Person Phone	
Person Fax	
Person Address	Genome Science Division, Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo, 153-8904, Japan	
Person Affiliation	The University of Tokyo	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2009-01-13	
PubMed ID	
Publication DOI	
Publication Author List	
Publication Title	
Publication Status	
Publication Status Term Source REF	
Experiment Description	The immune system sustains inflammation, and can occasionally inhibit tumor development. Conversely, chronic inflammation positively contributes to the development of cancer. Furthermore, it was revealed that the large number of macrophages and haematopoietic progenitor cells accumulate in response to primary tumors in pre-metastatic lungs. However, the relationship between the inflammation-like state induced by primary tumors in pre-metastatic phase and the migration of tumor cells in metastasis is not well understood. Here we analyzed transcriptional change of the lungs by pre-metastatic stimuli. Experiment Overall Design: We examined the lungs derived from mice that had been injected with tumor cells into the back subcutaneously. This model mimics pre-metastatic lungs responding to a primary tumor before tumor cells reach the lungs.	
Protocol Name	P-G5778-2	P-G5778-1	P-G5778-4	P-G5778-5	P-G5778-3	
Protocol Type	specified_biomaterial_action	grow	nucleic_acid_extraction	nucleic_acid_extraction	labeling	
Protocol Description	See Materials and Methods in the text by Hiratsuka and co-workers.	See Materials and Methods in the text by Hiratsuka and co-workers.	Total RNA were isolated from frozen tissues by TRIzol reagent.	Total RNA samples (lungs from B16-, LLC-bearing and normal mouse) were isolated from frozen tissues by TRIzol reagent.	Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets according to standard Affymetrix's procedure.	
Protocol Parameters	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF	
SDRF File	E-GEOD-5778.sdrf.txt	
Term Source Name	NCBI Taxonomy		The MGED Ontology	ArrayExpress	EFO	
Term Source File	http://www.ncbi.nlm.nih.gov/Taxonomy/		http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	
Term Source Version