Comment[ArrayExpressAccession]	E-GEOD-57050
MAGE-TAB Version	1.1						
Public Release Date	2014-04-25
Investigation Title	miRNA Profile in Normal and Preeclamptic Placenta						
Comment[Submitted Name]	miRNA Profile in Normal and Preeclamptic Placenta
Experiment Description	The purpose of this study was to determine the difference of the miRNA profiles between normal and preeclamptic placenta. Ten placental samples were analyzed.  Six were from preeclamptic patients and four were from normal pregnancies.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Wang	Wang					
Person First Name	Yuping	Yuping					
Person Email	ywang1@lsuhsc.edu						
Person Affiliation	LSUHSC-Shreveport						
Person Phone	318-675-5379						
Person Address	OB/GYN, LSUHSC-Shreveport, 1501 Kings Hwy, Shreveport, LA, USA						
Person Roles	submitter						
Protocol Name	P-GSE57050-1	P-GSE57050-5	P-GSE57050-6	P-GSE57050-2	P-GSE57050-3	P-GSE57050-4	P-GSE57050-7
Protocol Description	The data were analyzed with Expression Console 1.2 using RMA + DABG as the normalization method. ID_REF =  VALUE = Linear RMA Signal	Biotinylated template was prepared according to the standard Affymetrix protocol from 1 ug total RNA (FlashTag Biotin HSR RNA Labeling Kit, Affymetrix).	Following fragmentation, 1 ug of labeled product was hybridized for 18 hr at 48° C on GeneChip miRNA 2.0 arrays . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.	N/A	N/A	Trizol was used to extract total RNA.	GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	GROUP						
Experimental Factor Type	group						
Comment[SecondaryAccession]	GSE57050						
Comment[GEOReleaseDate]	2014-04-25						
Comment[ArrayExpressSubmissionDate]	2014-04-24						
Comment[GEOLastUpdateDate]	2014-04-26						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-57050.sdrf.txt