Comment[ArrayExpressAccession]	E-GEOD-56620
MAGE-TAB Version	1.1					
Public Release Date	2014-04-10
Investigation Title	Mouse utero-tubular junction (UTJ): 1: females in estrous no mating; 2: females in estrous mated with vasectomized male; 3: females in estrous mated with normal male: 1 vs. 2; 1 vs. 3; 2 vs. 3.					
Comment[Submitted Name]	Mouse utero-tubular junction (UTJ): 1: females in estrous no mating; 2: females in estrous mated with vasectomized male; 3: females in estrous mated with normal male: 1 vs. 2; 1 vs. 3; 2 vs. 3.
Experiment Description	Gene expression profiles comparing controls (1) with females mated with vasectomized male (2) or normal males (3). Three-condition experiment:  1 vs. 2; 1 vs. 3; 2 vs. 3. RNA source: 1. pool of 10 females control; 2. pool of 10  females in estrous mated with vasectomized male; 3. pool of 10 females in estrous mated with normal male.					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Kondoh	Kondoh	Watanabe			
Person First Name	Gen	Gen	Hitomi			
Person Email	kondohg@frontier.kyoto-u.ac.jp					
Person Affiliation	Kyoto University					
Person Phone	+81-75-751-4860					
Person Address	Institute for Frontier Medical Sciences, Kyoto University, 53 Syogoin-Kawahara-cho, Sakyo-ku, Kyoto, Kyoto, Japan					
Person Roles	submitter					
Protocol Name	P-GSE56620-1	P-GSE56620-4	P-GSE56620-5	P-GSE56620-2	P-GSE56620-3	P-GSE56620-6
Protocol Description	We used Agilent Feature Extraction Ver.9.5.3 for data analysis.  Background was subtracted and Processed Signal intensities were spatially detrended. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded. ID_REF =  VALUE = Normalized signal intensity Flag =  RawData = 	10 µg of total RNA were primed with 2 µl of 100 µM Oligo-dT primer at 70°C for 10 min, reversed transcribed at 42°C for 1 h in the presence of MMTV-RT, and then produced cRNA with T7 RNA polymerase with 100 µM NTP and 25 µM Cy3-labeled CTP.	see http://www.chem.agilent.com/scripts/generic.asp?lpage=11617&indcol=N&prodcol=Y	Total RNA samples were prepared from uterotubular junction tissues of ten CD-1 females with three different statuses: 1. estrous without mating.2. estrous mated with vasectomized males. 3. estrous mated with normal males.	Total RNA extracted using Trizol following manufacturer's instructions	We used Agilent DNA Microarray Scanner (G2505B) using one color scan setting.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	MATING					
Experimental Factor Type	mating					
Comment[SecondaryAccession]	GSE56620					
Comment[GEOReleaseDate]	2014-04-10					
Comment[ArrayExpressSubmissionDate]	2014-04-08					
Comment[GEOLastUpdateDate]	2014-04-10					
Comment[AEExperimentType]	transcription profiling by array					
Comment[AdditionalFile:Data1]	GSE56620_All_data2.xls					
SDRF File	E-GEOD-56620.sdrf.txt