Investigation Title	Transcription profiling of 2 cell mouse embryos of BRG1 mice and wild type mice to understand the effects of BRG1 on zygotic genome activation.	
Comment[Submitted Name]	Maternal BRG1 regulates zygotic genome activation in the mouse	
Experimental Design	individual_genetic_characteristics_design	co-expression_design	transcription profiling by array	
Experimental Design Term Source REF		mo	EFO	
Comment[ArrayExpressReleaseDate]	2007-11-13	
Comment[SecondaryAccession]	GSE5371	
Comment[AEMIAMESCORE]	3	
Comment[SecondaryAccession]	GDS2156	
Comment[ArrayExpressAccession]	E-GEOD-5371	
Comment[MAGETAB TimeStamp_Version]	2010-08-06 16:03:50 Last Changed Rev: 13058	
Experimental Factor Name	Genotype	
Experimental Factor Type	genotype	
Experimental Factor Term Source REF	
Person Last Name	Pan	
Person First Name	Hua	
Person Mid Initials	
Person Email	huapan@sas.upenn.edu	
Person Phone	
Person Fax	
Person Address	Department of Biology, University of Pennsylvania, 415 S University Avenue , Philadelphia, 19104, USA	
Person Affiliation	University of Pennsylvania	
Person Roles	submitter	
Person Roles Term Source REF		
Quality Control Type	
Quality Control Term Source REF	
Replicate Type	
Replicate Term Source REF	
Normalization Type	
Normalization Term Source REF	
Date of Experiment	
Public Release Date	2007-11-13	
PubMed ID	16818606	
Publication DOI	16818606	
Publication Author List	Scott J Bultman, Thomas C Gebuhr, Hua Pan, Petr Svoboda, Richard M Schultz, Terry Magnuson	
Publication Title	Maternal BRG1 regulates zygotic genome activation in the mouse.	
Publication Status	journal_article	
Publication Status Term Source REF	The MGED Ontology	
Experiment Description	Embryos were collected at 2 cell stage.  cRNA from four biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430 v2. Experiment Overall Design: 4 embryo at 2 cell stage biological replicates were analyzed.	
Protocol Name	P-G5371-1	P-G5371-2	P-G5371-3	P-G5371-4	
Protocol Type	nucleic_acid_extraction	labeling	hybridization	image_acquisition	
Protocol Description	Total RNA from 20 embryos was extracted and purified using an Picopure kit (Arcturus).	total RNA from 20 embryos was processed to produce biotinylated cRNA targets using Affy Small Sample Target Labeling Assay Version II.	standard Affymetrix procedures	standard Affymetrix procedures	
Protocol Parameters	
Protocol Hardware	
Protocol Software	
Protocol Contact	
Protocol Term Source REF	The MGED Ontology	
SDRF File	E-GEOD-5371.sdrf.txt	
Term Source Name		The MGED Ontology	ncbitax	NCI_thesaurus	ArrayExpress	mo	EFO	The MGED Ontology	
Term Source File		http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ncbi.nlm.nih.gov/Taxonomy/taxonomyhome.html	ncithesaurus.obo.alt	http://www.ebi.ac.uk/arrayexpress	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/efo/	http://mged.sourceforge.net/ontologies/MGEDontology.php	
Term Source Version