Comment[ArrayExpressAccession] E-GEOD-53379 MAGE-TAB Version 1.1 Public Release Date 2015-02-27 Investigation Title Phenotype specific analyses of p53 reveal distinct regulatory mechanism for chronically activated p53 Comment[Submitted Name] Phenotype specific analyses of p53 reveal distinct regulatory mechanism for chronically activated p53 Experiment Description microarray analysis with and without sh-p53 in IMR90 (normal human diploid fiborblasts) Phenotypes were created by overexpression of RasG12V, E1A/RasG12V in IMR90 cells, in growing IMR90, inr quiescent IMR90 cells (3 days confluence) and 24 or 48 hours Etoposide treated IMR90 cells were subjected to lentiviral knockdown of p53 or vector Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Menon Kirschner Menon Person First Name Suraj Kristina Suraj Person Email suraj.menon@cruk.cam.ac.uk Person Affiliation Cambridge Research Institute, Cancer Research UK Person Address Cambridge Research Institute, Cancer Research UK, Li Ka Shing Center, Robinson Way, Cambridge, United Kingdom Person Roles submitter Protocol Name P-GSE53379-1 P-GSE53379-5 P-GSE53379-6 P-GSE53379-2 P-GSE53379-3 P-GSE53379-4 P-GSE53379-7 Protocol Description Raw intensity data from the array scanner was processed using the BASH and HULK algorithms as implemented in the beadarray package. Log2 transformation and quantile normalization of the data were performed across all sample groups. ID_REF = VALUE = Log2 quantile normalised data Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays Standard Illumina hybridization protocol 24 or 48 hours Etoposide (100microM),pBabe-Puro H-RasG12V, pWXL-Hygro E2A, H-RasG12V, pLNCX-Neo ER:H-RasG12V overexpression, conflunecy 3 days cultured in 5%O2 in DMEM with 10% FBS and Pen/Strep and Glutamine Qiagen RNeasy protocol Standard Illumina scanning protocol Protocol Type normalization data transformation protocol labelling protocol hybridization protocol sample treatment protocol growth protocol nucleic acid extraction protocol array scanning protocol Experimental Factor Name sample group Experimental Factor Type sample group Comment[SecondaryAccession] GSE53379 Comment[GEOReleaseDate] 2015-02-27 Comment[ArrayExpressSubmissionDate] 2013-12-16 Comment[GEOLastUpdateDate] 2015-02-28 Comment[AEExperimentType] transcription profiling by array Comment[AdditionalFile:Data1] GSE53379_non_normalized.txt SDRF File E-GEOD-53379.sdrf.txt