Comment[ArrayExpressAccession]	E-GEOD-52702
MAGE-TAB Version	1.1						
Public Release Date	2013-11-30
Investigation Title	Interactive effects of arsenic and a high-fat diet on hepatic gene expression						
Comment[Submitted Name]	Interactive effects of arsenic and a high-fat diet on hepatic gene expression
Experiment Description	Chronic exposure to inorganic arsenic (iAs) or a high-fat diet (HFD) can produce liver injury. However, the interactive molecular biological effects and mechanism of iAs and HFD are as of yet unclear. We used microarrays to detail the interactive effects of arsenic and a high-fat diet on hepatic gene expression. The C57BL/6 Mice fed low-fat diet (LFD) or HFD were exposed to 3 mg/L iAs or deionized water for 10 weeks. Then, hepatic RNA were extraction and hybridization on Affymetrix microarrays. Differentially expressed genes in LFD+As, HFD, and HFD+As groups compared to LFD group were identified, and interactive molecular biological effects and mechanism of iAs and HFD were discussed.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Wu	Wu	Liu				
Person First Name	Bing	Bing	Su				
Person Email	bwu@nju.edu.cn						
Person Affiliation	Nanjing University						
Person Address	School of the Environment, Nanjing University, NO.163 Xianlin Road, Nanjing, Jiangsu, China						
Person Roles	submitter						
Protocol Name	P-GSE52702-1	P-GSE52702-5	P-GSE52702-6	P-GSE52702-2	P-GSE52702-3	P-GSE52702-4	P-GSE52702-7
Protocol Description	Affymetrix Expression Console software (v.1.0) was applied to provides signal estimation and QC functionality ID_REF =  VALUE = Log2 RMA signal	Biotinylated cRNA were prepared according to the standard Affymetrix protocol	Following fragmentation, cRNA were hybridized in GeneChip Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450	A total of forty mice were randomly assigned to four groups. Mice were fed a control LFD (13% fat in calories) or HFD (42% fat in calories) and given deionized water with or without sodium arsenite (3 mg iAs/L) for 10 weeks.	Male C57Bl/6J mice were housed in stainless-steel cages under controlled conditions (25±3 °C, 50±5 % humidity and 12/12 h light/dark cycle) for 10 weeks	Total RNA for each mouse was separately extracted using Trizol reagent protocol.10 mice per group were randomly divided into two sets (5 mice per set). Equal amounts of RNAs from 5 mice in the same set were pooled into one sample, and two samples per group were obtained.	GeneChips were scanned using the GeneChip Scanner 3000.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	COMPOUND	GROWTH PROTOCOL					
Experimental Factor Type	compound	growth protocol					
Comment[SecondaryAccession]	GSE52702						
Comment[GEOReleaseDate]	2013-11-30						
Comment[ArrayExpressSubmissionDate]	2013-11-25						
Comment[GEOLastUpdateDate]	2013-11-30						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-52702.sdrf.txt