Comment[ArrayExpressAccession]	E-GEOD-52522
MAGE-TAB Version	1.1								
Public Release Date	2013-11-20
Investigation Title	Transcriptional program for induced delta cell on day10								
Comment[Submitted Name]	Transcriptional program for induced delta cell on day10
Experiment Description	Direct lineage conversion of adult cells is a promising approach for regenerative medicine.  A major challenge of lineage conversion is to generate specific subtypes of cells, closely related cells with distinct properties.  The pancreatic islets contain three major hormone-secreting endocrine subtypes:  insulin+ β-cells, glucagon+ α-cells, and somatostatin+ δ-cells.  We previously reported that a combination of three transcription factors, Ngn3, Mafa, and Pdx1, directly reprogram pancreatic acinar cells to β-cells.  We now show that acinar cells can be converted to δ-like and α-like cells by Ngn3 and Ngn3+Mafa respectively.  Thus, three major islet endocrine subtypes can be derived by acinar reprogramming.  Ngn3 promotes establishment of a generic endocrine state in acinar cells at the onset of reprogramming in addition to promoting δ-specification.  Mafa and Pdx1 suppress δ-specification in α- and β-cell formation.  These studies identify a set of defined factors whose combinatorial actions reprogram acinar cells to distinct islet endocrine subtypes in vivo. induced beta cells samples at day 10 collected for the microarray								
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl							
Person Last Name	Li	Li	Zhou						
Person First Name	Weida	Weida	Qiao						
Person Email	weidali@mcb.harvard.edu								
Person Affiliation	Harvard University								
Person Phone	6174961687								
Person Address	Harvard University, 7 Divninity Avenue, Cambridge, MA, USA								
Person Roles	submitter								
Protocol Name	P-GSE52522-2	P-GSE52522-3	P-GSE52522-1	P-GSE52522-7	P-GSE52522-8	P-GSE52522-4	P-GSE52522-5	P-GSE52522-6	P-GSE52522-9
Protocol Description	The raw signal was  quantile-normalized across samples in R. ID_REF =  VALUE = quantile-normalized signal idelta-2.Detection Pval = 	The raw signal was  quantile-normalized across samples in R. ID_REF =  VALUE = quantile-normalized signal idelta-3.Detection Pval = 	The raw signal was  quantile-normalized across samples in R. ID_REF =  VALUE = quantile-normalized signal idelta-1.Detection Pval = 	label protocol for Illumina MouseRef-8 v2.0 expression beadchip	Hyb protocol for Illumina MouseRef-8 v2.0 expression beadchip	Pancreata were perfused through the common bile duct. After removing endogenous islets, the acinar fraction was digested with liberase and Elastase. Roche) into single cells.	Rag1-/- were obtained from Jackson Labs. For delta- cell induction, 100ul (>1x 109 pfu) of purified adenovirus was directly injected into the splenic lobe of the dorsal pancreas.	Cherry+ induced beta cells were isolated by fluorescent activated cell sorting (FACS) with FACSaria (BD Bioscience), RNA samples were extracted by Trizol	Scan protocol for Illumina MouseRef-8 v2.0 expression beadchip
Protocol Type	normalization data transformation protocol	normalization data transformation protocol	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Comment[SecondaryAccession]	GSE52522								
Comment[GEOReleaseDate]	2013-11-20								
Comment[ArrayExpressSubmissionDate]	2013-11-19								
Comment[GEOLastUpdateDate]	2013-11-20								
Comment[AEExperimentType]	transcription profiling by array								
Comment[AdditionalFile:Data1]	GSE52522_non-normalized.txt								
SDRF File	E-GEOD-52522.sdrf.txt