Investigation Title	Transcription profiling of human healthy peripheral blood cells isolated from male participants at rest	
Comment[Submitted Name]	gene expression profiles of healthy male participants in rest	
Experimental Design	unknown_experiment_design_type	transcription profiling by array	
Experimental Design Term Source REF		EFO	
Comment[ArrayExpressReleaseDate]	2008-06-13	
Comment[SecondaryAccession]	GSE5237	
Comment[AEMIAMESCORE]	3	
Comment[ArrayExpressAccession]	E-GEOD-5237	
Comment[MAGETAB TimeStamp_Version]	2010-08-06 16:06:22 Last Changed Rev: 13058	
Experimental Factor Name	
Experimental Factor Type	
Experimental Factor Term Source REF	
Person Last Name	Mosig	
Person First Name	Sandy	
Person Mid Initials	
Person Email	sandy.mosig@mti.uni-jena.de	
Person Phone	
Person Fax	
Person Address	Molecular Hemostaseology - Institute of Vascular Medicine, University Hospital Jena, Bachstrasse 18, Jena, 07743, Germany	
Person Affiliation	University Hospital Jena	
Person Roles	submitter	
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Normalization Type	
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Date of Experiment	
Public Release Date	2008-06-13	
PubMed ID	
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Experiment Description	These gene expression profiles were measured to create a broad and balanced control for any project that examines gene expression changes in men exposed to a defined stimulus (see series 5105).  Experiment Overall Design: 9 healthy men (aged 21 to 44) gave written agreement to participate to this study. At the time of blood withdrawal all participants were non-smokers, with normal BMI and did not obtain any medicamentation. We collected 12ml of whole blood and isolated T-lymphocytes out of 4ml and monocytes out of 8ml whole blood.	
Protocol Name	P-G5237-1	P-G5237-4	P-G5237-3	P-G5237-2	
Protocol Type	specified_biomaterial_action	specified_biomaterial_action	nucleic_acid_extraction	labeling	
Protocol Description	8ml of whole blood were collected in BD Vacutainer CPT tubes. 200 Âμl of RosetteSep Tcell Enrichment cocktail were added immediately following the blood withdrawal. Tubes were inverted five times and incubated for 20 minutes at room temperature. Tubes were next centrifuged for 20 minutes at 1850g at room temperature. The cells were washed two times in icecold (4°C) PBS/ 2mM EDTA once in a volume of 50ml and the second time with a final volume of 5ml, spun down for 10 minutes at 500g at 4°C, the supernatant was removed by vacuum suction and the final pellet was lysed with Trizol.	4ml of whole blood were collected in BD Vacutainer CPT tubes. 200 Âμl of RosetteSep Tcell Enrichment cocktail were added immediately following the blood withdrawal. Tubes were inverted five times and incubated for 20 minutes at room temperature. Tubes were next centrifuged for 20 minutes at 1850g at room temperature. The cells were washed two times in icecold (4°C) PBS/ 2mM EDTA once in a volume of 50ml and the second time with a final volume of 5ml, spun down for 10 minutes at 500g at 4°C, the supernatant was removed by vacuum suction and the final pellet was lysed with Trizol.	RNA was extracted from Trizol through addition of chloroform and phase separation using PLG tubes. Additionally RNA was cleaned up using the Rneasy Micro Kit with a Dnase step.	biotinylated RNA was prepared from 1 Âμg total RNA according to the Affymetrix manual (2005)	
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SDRF File	E-GEOD-5237.sdrf.txt	
Term Source Name	mo	The MGED Ontology	ArrayExpress	EFO	
Term Source File	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://mged.sourceforge.net/ontologies/MGEDontology.php	http://www.ebi.ac.uk/arrayexpress	http://www.ebi.ac.uk/efo/	
Term Source Version