Comment[ArrayExpressAccession]	E-GEOD-51908
MAGE-TAB Version	1.1						
Public Release Date	2013-10-31
Investigation Title	miRNA expression data from normal and Malignant hematopoietic cells						
Comment[Submitted Name]	miRNA expression data from normal and Malignant hematopoietic cells
Experiment Description	This data was used to identify miRNA signatures that can be used to calssify specific leukemia types including AML, precursor B ALL, precursor T ALL, and normal hematopoietic progenitor and mature populations This data was used to identify miRNA signatures that can be used to calssify specific leukemia types including AML, precursor B ALL, precursor T ALL, and normal hematopoietic progenitor and mature populations AML cell lines and patient samples, B ALL cell lines and Patient samplest, T ALL cell lines and patient samples, norma B cells, normal granulocytes, normal monocytes, normal T cells and normal CD34+ cells were used for RNA extraction and hybridization on Affymetrix microarrays. All the AML, B ALL, T ALL cell lines were cultured in vitro under appropriate culture conditons and harvested in their log phase growth for RNA extraction. AML, B ALL, and T ALL patient samples were collected...(I assume these are the PBMCs from eitehr peripheral blood or bone marrow from patients, please confirm). Normal B cells, granulocytes, monocytes, and T cells were purified from human peripheral blood of normal healthy donors.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Shetty	Civin					
Person First Name	Amol	Curt					
Person Mid Initials	Carl	I					
Person Email	ashetty@som.umaryland.edu						
Person Affiliation	University of Maryland Baltimore						
Person Address	Institute for Genome Sciences, University of Maryland Baltimore, 801 W. Baltimore Street, Baltimore, Maryland, USA						
Person Roles	submitter						
Protocol Name	P-GSE51908-1	P-GSE51908-5	P-GSE51908-6	P-GSE51908-2	P-GSE51908-3	P-GSE51908-4	P-GSE51908-7
Protocol Description	The data were analyzed using the Partek Genomic Suite 6.6  to load the data before using the RMA algorithem to normalize all of the data ID_REF =  VALUE = RMA signal intensity (human only)	About 500 ng of total RNA was biotin labeled using Affymetrix propritary FlashTag Biotin HSR RNA labeling Kit that is covalently linked to biotin.	Biotin labled RNA was hybridized for 16 hr at 45C on GeneChip miRNA 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.	N/A	All the leukemia cell lines were culture in vitro under appropriate cell cutlure conditions according to the supplier recommondataion. Normal mature hematopoietic cells were purifed from normal healthy donor peripheral blood using Ficoll gradiant centrifugation followed by FACS sorting.	Cell were lysed in Trizol and total RNA was purifed using miRNAeasy kit according to the manufacturer's instructions.	GeneChips were scanned using Genechip Scanner 3000 and software AffyMetrix Genechip Command Console .
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	CELL LINE	CELL TYPE	PHENOTYPE	ORGANISM PART			
Experimental Factor Type	cell line	cell type	phenotype	organism part			
Comment[SecondaryAccession]	GSE51908						
Comment[GEOReleaseDate]	2013-10-31						
Comment[ArrayExpressSubmissionDate]	2013-10-30						
Comment[GEOLastUpdateDate]	2013-11-02						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-51908.sdrf.txt