Comment[ArrayExpressAccession] E-GEOD-51767 MAGE-TAB Version 1.1 Public Release Date 2014-02-18 Investigation Title Construction of gene expression profiles for anoxia-reoxygenation in Arabidopsis Comment[Submitted Name] Construction of gene expression profiles for anoxia-reoxygenation in Arabidopsis Experiment Description To understand how plants respond to anoxia-reoxygenation, we have employed a global microarray expression profiling as a basic platform to characterize genes with the potential to mediate the recovery responses in Arabidopsis. 7-day-old seedlings were trated with 4hr and 8hr anoxia, and then reoxygenated in air for 0, 0.5, 1, 3, 6 hrs. Genes changed in both condition were designated as reoxygenation-regulated genes. They included the genes in ROS detoxification, dehydration, metabolic process, and many other responses. Interestingly, ethylene was also involved in this recovery process. We further adopted ein2-5 and ein3eil1 microarray to investigate ethylene signaling. Our results showed ethylene partially regulate reoxygenation regulated genes and is reruired for plant survival in reoxygenation. Genes expression during anoxia-reoxygenation in Arabidopsis seedlings was measured at 0, 0.5, 1, 3, 6 hours after anoxia treatment, and normal condition before anoxia. Three independent experiments were performed at each time (Nor, 0, 0.5, 1, 3, 6) under 4hr anoxia-reoxygenation condition by using Col-0, ein2-5, and ein3eil1. Four independent experiments were performed at each time (Nor, 0, 0.5, 1, 3, 6) under 8hr anoxia-reoxygenation condition by using Col-0. Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Tsai Tsai Person First Name Kuen-Jin Kuen-Jin Person Email aletent99@gmail.com Person Affiliation Academia Sinica, Taiwan Person Phone +886-2-2787-2039 Person Address Agricultural Biotechnology Research Center, Academia Sinica, Taiwan, 128 Academia Road, Section 2, Nankang, Taipei, Taiwan Person Roles submitter Protocol Name P-GSE51767-1 P-GSE51767-5 P-GSE51767-6 P-GSE51767-2 P-GSE51767-3 P-GSE51767-4 P-GSE51767-7 Protocol Description The raw data of hybridization was imported into the analysis software GeneSpring 11.5 (Agilent Technologies), and the data of normaxia of Col-0 was used as control to normalize all the samples. threshold raw signals to: 1.0; normalization algorithm: Percentile Shift; Percentile target: 75; no baseline transformation or filtering ID_REF = VALUE = log2 normalized Cy3 labeling (CyDye, Agilent Technologies, USA) was performed during the in vitro transcription process. 1.65 M-NM-