Comment[ArrayExpressAccession]	E-GEOD-51574
MAGE-TAB Version	1.1			
Public Release Date	2015-06-16
Investigation Title	Small RNA Solexa sequencing of mouse CD11c+Ia high and CD11c+Ia low cells			
Comment[Submitted Name]	Small RNA Solexa sequencing of mouse CD11c+Ia high and CD11c+Ia low cells
Experiment Description	We applied Illumina massively parallel signature sequencing to identify miRNomes in CD11c+Ia high and CD11c+Ia low cells.The miRNomes of these DC subsets will contribute to investigate the significance of miRNAs in DC immunobiology. Examination of miRNome in CD11c+Ia high and CD11c+Ia low cells . All two mouse cell types.			
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl		
Person Last Name	Su	Su	Cao	
Person First Name	Xiaoping	Xiaoping	Xuetao	
Person Email	bloooge@163.com			
Person Affiliation	National Key Laboratory of Medical Immunology & Institute of Immunology			
Person Address	National Key Laboratory of Medical Immunology & Institute of Immunology, 800 Xiangyin Road, Shanghai, China			
Person Roles	submitter			
Protocol Name	P-GSE51574-4	P-GSE51574-1	P-GSE51574-3	P-GSE51574-2
Protocol Description	Sequencing data alignment were mainly analyzed using SOAP (Short Oligonucleotide Alignment Program) (http://soap.genomics.org.cn) algorithm. Then the image files generated by the sequencer were processed to produce digital-quality data. The subsequent procedures performed with Solexa were summarizing data production, evaluating sequencing quality, calculating length distribution of small RNA reads and filtrating reads contaminated by rRNA, tRNA, mRNA, snRNA, and snoRNA. Finally, clean reads were compared with a miRBase database (release 19.0) and the copy numbers of each unique sequence were calculated. Supplementary_files_format_and_content: Text files containing clean tags, 18-30 nt, tab delimited, length | number | sequence	Total RNA, containing miRNA, was extracted using mirVana miRNA Isolation Kit (Ambion) and passed the RNA quality control for Solexa sequencing.	Briefly, after PAGE purification of small RNA molecules under 30 bases and ligation of a pair of Solexa adaptors to their 5' and 3' ends, the small RNA molecules were amplified using the adaptor primers for 12 cycles and the fragments around 90 bp (small RNA+adaptors) were isolated from agarose gel. The purified DNA was used directly for cluster generation and sequencing analysis using the Illumina's Solexa Sequencer according to the manufacturer's instructions.	To purify CD11c+Ia high and CD11c+Ia low cells, CD11c+ cells enriched by CD11c-conjugated microbeads from spleen were stained with fluorescently labeled anti-Ia-FITC, and then CD11c+Ialow cells and CD11c+Iahigh cells were sorte using a MoFlo XDP cell sorter (Beckman Coulter), the purity of which was confirmed to be >95%.
Protocol Type	normalization data transformation protocol	sample treatment protocol	nucleic acid library construction protocol	growth protocol
Publication Title	miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1.			
Publication Author List	Su X, Qian C, Zhang Q, Hou J, Gu Y, Han Y, Chen Y, Jiang M, Cao X			
PubMed ID	24309499			
Publication DOI	10.1038/ncomms3903			
Comment[SecondaryAccession]	GSE51574			
Comment[GEOReleaseDate]	2015-06-16			
Comment[ArrayExpressSubmissionDate]	2013-10-23			
Comment[GEOLastUpdateDate]	2015-06-16			
Comment[AEExperimentType]	RNA-seq of non coding RNA			
Comment[SecondaryAccession]	SRP031845			
Comment[SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/SRR1015485-SRR1015486			
SDRF File	E-GEOD-51574.sdrf.txt