Comment[ArrayExpressAccession]	E-GEOD-51102
MAGE-TAB Version	1.1						
Public Release Date	2013-09-24
Investigation Title	Autoimmune antigen and serum factor antigen reactivity in a cohort of patients with mycobacterium infection shown previously to be positive and negative for IgG autoantibodies to IFN-gamma.						
Comment[Submitted Name]	Autoimmune antigen and serum factor antigen reactivity in a cohort of patients with mycobacterium infection shown previously to be positive and negative for IgG autoantibodies to IFN-gamma.
Experiment Description	The aim of the study was to test wether our microarray platform is capable of detecting previously described IgG autoantibodies to IFN-gamma. We diluted patient sera 1:250 and incubated dilutions on a nitrocellulose-platform array printed with whole protein antigens. In this study, serum from 10 individuals (subsetted into two groups: individuals positive or negative for previously described IFN-gamma-targeted autoantibodies) was profiled for IgG autoantibody reactivity to whole protein autoimmune and serum factor antigens. We found that IFN-gamma-targeted IgG autoantibodies were recognized in the IFNg-pos samples and also identified other array targets significantly associated with anti-IFNg positive individials using the significance analysis of microarrays (SAM) algorithm.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Price	Price	Utz				
Person First Name	Jordan	Jordan	Paul				
Person Mid Initials		V	J				
Person Email	geo@ncbi.nlm.nih.gov						
Person Affiliation	Stanford University						
Person Address	Stanford University, CCSR 2250, Stanford, CA, USA						
Person Roles	submitter						
Protocol Name	P-GSE51102-1	P-GSE51102-5	P-GSE51102-6	P-GSE51102-2	P-GSE51102-3	P-GSE51102-4	P-GSE51102-7
Protocol Description	Array images were analyzed using GenePix 6.0 software (Molecular Devices) ID_REF =  VALUE = Median Fluorescence intensity (MFI) minus local background	N/A	Serum was diluted 1:250 from stocks (maintained at -80 deg. C. in) in PBS + 0.5% Tween 20 (Sigma-Aldrich, St. Louis, MO) and 2.5% fetal calf serum (Omega Scientific, Tarzana CA). Post primary incubation, washed arrays were incubated with 0.375ug/ml Goat anti-Human IgG conjugated to Cy5 (Jackson Immunoresearch, West Grove, PA).	N/A	N/A	N/A	Arrays were scanned using an Axon digital scanning system (Molecular Devices, Sunnyvale, CA)
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	ANTI-IFN-GAMMA						
Experimental Factor Type	anti-ifn-gamma						
Comment[SecondaryAccession]	GSE51102						
Comment[GEOReleaseDate]	2013-09-24						
Comment[ArrayExpressSubmissionDate]	2013-09-23						
Comment[GEOLastUpdateDate]	2013-09-26						
Comment[AEExperimentType]	other						
SDRF File	E-GEOD-51102.sdrf.txt