Comment[ArrayExpressAccession]	E-GEOD-50880
MAGE-TAB Version	1.1						
Public Release Date	2013-09-17
Investigation Title	Expression data from preadipocytes isolated from laying hen						
Comment[Submitted Name]	Expression data from preadipocytes isolated from laying hen
Experiment Description	We used Affymetrix Chicken Genome Array to identify transcripts differentially expressed between cells treated with DMIOA and control cells and between DMIOA and DMIOA+20S treated cells Preadipocytes isolated from laying hen were treated with DMIOA and DMIOA+20S to identify transcripts differentailly expressed between control cells and DMIOA treated and between DMIOA and DMIOA+20S treated cells						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Hunde	Regassa	Kim				
Person First Name	Alemu	Alemu	Woo-Kyun				
Person Mid Initials	Regassa						
Person Email	aregassa2005@gmail.com						
Person Affiliation	University of Manitoba						
Person Phone	1-204-430-1308						
Person Address	Animal Science, University of Manitoba, 12 Dafoe Road, Winnipeg, Manitoba, Canada						
Person Roles	submitter						
Protocol Name	P-GSE50880-1	P-GSE50880-5	P-GSE50880-6	P-GSE50880-2	P-GSE50880-3	P-GSE50880-4	P-GSE50880-7
Protocol Description	Data analysis, background correction and normalisation were performed using GC-RMA ID_REF =  VALUE = Log2 GC-RMA signal intensity	Biotinylated aRNA were prepared according to the standard Affymetrix protocol from 15 ug total RNA (GeneChip 3' IVT Express Kit, Affymetrix).	Following fragmentation, 12.5 ug of aRNA were hybridized for 16 hr at 45C on GeneChip Chicken Genome Array. GeneChips were washed and stained using the Affymetrix Fluidics Station 450.	Preadipocytes were treated with an adipogenic cockatail (DMIOA ) and (DMIOA+20S) and non-treated control cells were included for comparison purpose.	Preadipocytes were isolated from laying hen and cultured in 10 mL Dulbecco's Modified Eagle's Medium (DMEM).	Trizol extraction of total RNA was performed according to the manufacturer's instructions.	GeneChips were scanned using GeneChip™ 3000 laser confocal slide scanner
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Comment[SecondaryAccession]	GSE50880						
Comment[GEOReleaseDate]	2013-09-17						
Comment[ArrayExpressSubmissionDate]	2013-09-16						
Comment[GEOLastUpdateDate]	2013-09-19						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-50880.sdrf.txt