Comment[ArrayExpressAccession]	E-GEOD-49998				
MAGE-TAB Version	1.1				
Public Release Date	2014-04-21				
Investigation Title	Gene Level Expression Profiling: Activity dependent genes in the olfactory epithelium				
Comment[Submitted Name]	Gene Level Expression Profiling: Activity dependent genes in the olfactory epithelium				
Experiment Description	Gene level analysis of RNA samples from mice olfactory epithelium Gene expression profiling in the olfactory epithelium was performed to obtain a better understanding of the processes mediating activity dependent gene regulation We analyzed total RNA from olfactory epithelium tissue from 3 mice at 5 days following unilateral naris occlusion (NO) using the Affymetrix Mouse Exon 1.0 ST Array, comparing unilateral and ipsilateral epithelia.				
Term Source Name	ArrayExpress	EFO			
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl			
Person Last Name	McClintock	Fischl	Heron	Stromberg	McClintock
Person First Name	Timothy	Adrian	Paula	Arnold	Timothy
Person Mid Initials	Scott	M			S
Person Email	mcclint@uky.edu				
Person Affiliation	University of Kentucky College of Medicine				
Person Phone	(859) 323-1083				
Person Address	Physiology, University of Kentucky College of Medicine, 138 Leader Avenue, Lexington, KY, USA				
Person Roles	submitter				
Protocol Name	P-GSE49998-1	P-GSE49998-3	P-GSE49998-4	P-GSE49998-2	P-GSE49998-5
Protocol Description	Raw data were processed with the Exon Array Computational Tool (ExACT) (Affymetrix) for background correction and normalization. Data from the 10% of genes with the lowest average signal intensities were discarded. Significant differecenes were judged as mRNA had p < 0.05 one T test, and greater than a 25% difference in abundance. ID_REF =  VALUE = RMA signal	standard Affymetrix protocol	Samples were hybridized with GeneChip Mouse Exon 1.0 ST Arrays (Affymetrix) and scanned at the University of Kentucky Microarray Core Facility.	RNA was isolated from epithelial tissue homogenized in 700 µL TriReagent according to the manufacturer’s instructions. RNA purity was checked using an Agilent Bioanalyzer	Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	treatment				
Experimental Factor Type	treatment				
Publication Title	Activity-Dependent Genes in Mouse Olfactory Sensory Neurons.				
Publication Author List	Fischl AM, Heron PM, Stromberg AJ, McClintock TS				
PubMed ID	24692514				
Publication DOI	10.1093/chemse/bju015				
Comment[SecondaryAccession]	GSE49998				
Comment[GEOReleaseDate]	2014-04-21				
Comment[ArrayExpressSubmissionDate]	2013-08-19				
Comment[GEOLastUpdateDate]	2014-04-22				
Comment[AEExperimentType]	transcription profiling by array				
SDRF File	E-GEOD-49998.sdrf.txt