Comment[ArrayExpressAccession]	E-GEOD-49849
MAGE-TAB Version	1.1					
Public Release Date	2013-08-14
Investigation Title	Alterations in mRNA levels in the dentate gyrus in epileptic rats					
Comment[Submitted Name]	Alterations in mRNA levels in the dentate gyrus in epileptic rats
Experiment Description	Analysis of the dentate gyrus of amygdala electrical stimulation model of temporal lobe epilepsy. Results provide insight into the molecular mechanism underlying epileptogenesis. This study was designed to estimate changes in gene expression levels after 7 and 30 days after electrical stimulation of amygdala as a model of temporal lobe epilepsy. The advantage of this study is time matched control (sham operated animals sacrificed at the same age as stimulated animals).					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Lukasiuk	Bot	Debski	Lukasiuk		
Person First Name	Katarzyna	Anna	Konrad	Katarzyna		
Person Mid Initials			J			
Person Email	k.lukasiuk@nencki.gov.pl					
Person Affiliation	Nencki Institute of Experimental Biology Polish Academy of Sciences					
Person Address	Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology Polish Academy of Sciences, 3 Pasteur Street, Warsaw, Poland					
Person Roles	submitter					
Protocol Name	P-GSE49849-1	P-GSE49849-4	P-GSE49849-5	P-GSE49849-2	P-GSE49849-3	P-GSE49849-6
Protocol Description	R/Bioconductor RMA algorithm implemented in oligo package ver 1.22.0 ID_REF =  VALUE = log2 RMA signal	100 ng of total mRNA was used for cDNA synthesis using Ambion WT Expression Kit (Life Technologies #4411974).	Affymetrix guidelines for GeneAtlas instrument	Amygdala electrical stimulation model of temporal lobe epilepsy.	Total RNA including miRNA was isolated from the left dentate gyrus region from hippocampi isolated on ice and placed in RNAlater solution (Ambion, AM7024) and stored at -20C until use. Isolation from dissected left dentate gyrus was performed by miRNease Mini kit (QIAGEN, 217004), according to the manufacturer's instructions.	Affymetrix GeneAtlas System
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	TREATMENT	DAYS AFTER STIMULATION				
Experimental Factor Type	treatment	days after stimulation				
Comment[SecondaryAccession]	GSE49849					
Comment[GEOReleaseDate]	2013-08-14					
Comment[ArrayExpressSubmissionDate]	2013-08-13					
Comment[GEOLastUpdateDate]	2013-08-14					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-49849.sdrf.txt