Comment[ArrayExpressAccession] E-GEOD-49286 MAGE-TAB Version 1.1 Public Release Date 2013-07-28 Investigation Title Genome-wide analysis of c-Myb-responsive gene expression in prostate cancer cells Comment[Submitted Name] Genome-wide analysis of c-Myb-responsive gene expression in prostate cancer cells Experiment Description Analysis of c-Myb-regulation of gene expression. The hypothesis tested in the present study was that c-Myb influences the expression of specific sets of genes that are involved in cell cycle, DNA replication, recombination and repair. Results provide important information on c-Myb-responsive genes that may be crucial to the cell survival and the progression of prostate cancer. Total RNA obtained from c-Myb siRNA-transfected prostate cancer cells compared to negative control siRNA-transfected prostate cancer cells 48 h after siRNa transfection. Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name Thompson Thompson Li Chang Lee Lee Person First Name Timothy Timothy Likun Wenjun Hyun-Sung Ju-Seog Person Mid Initials C. C Person Email timthomp@mdanderson.org Person Affiliation MD Anderson Cancer Center Person Phone 7137929955 Person Address Genitourinary Medical Oncology, MD Anderson Cancer Center, 1515 Holcombe Blvd., Houston, Texas, USA Person Roles submitter Protocol Name P-GSE49286-1 P-GSE49286-3 P-GSE49286-4 P-GSE49286-2 P-GSE49286-5 Protocol Description The data were normalised using quantile normalisation with IlluminaGUI in R ID_REF = VALUE = quantile normalized signal Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit (Invitrogen). Standard Illumina hybridization protocol RNA was extracted and prepared with MirVana miRNA Isolation Kit (Invitrogen) in accordance with the prescribed protocol provided with the kit. Standard Illumina scanning protocol Protocol Type normalization data transformation protocol labelling protocol hybridization protocol nucleic acid extraction protocol array scanning protocol Experimental Factor Name CELL LINE GENOTYPE Experimental Factor Type cell line genotype Comment[SecondaryAccession] GSE49286 Comment[GEOReleaseDate] 2013-07-28 Comment[ArrayExpressSubmissionDate] 2013-07-27 Comment[GEOLastUpdateDate] 2013-07-29 Comment[AEExperimentType] transcription profiling by array Comment[AdditionalFile:Data1] GSE49286_non-normalized_data.txt SDRF File E-GEOD-49286.sdrf.txt