Comment[ArrayExpressAccession]	E-GEOD-48559
MAGE-TAB Version	1.1						
Public Release Date	2013-12-28
Investigation Title	Identification of MAX signature on MAX deficient SCLC cell lines						
Comment[Submitted Name]	Identification of MAX signature on MAX deficient SCLC cell lines
Experiment Description	To development our gene expression approach, we have employed whole genome microarray expression profiling as a discovery platform to identify genes  potentialy regulated by the transcription factor MAX.Human SCLC cell lines waere analyzed for mutations at the MAX locus. Those cell lines that were found mutated in MAX and showed no MAX protein expression were used as a models to restore the expression of MAX transcription factor, and Identify MAX signature on Human  SCLC. Human SCLC cell lines were hybridyzed in the following manner according for different conditions (Mock -Ø, MAX, shBRG1, MAX-shBRG1): 3 different biological samples for Mock, 3 different biological samples for MAX, 3 different samples for shBRG1 and 3 replicates of the same sample for MAX-shBRG1.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Gómez	Romero Ferraro	Torres Diz	Sánchez-Céspedes			
Person First Name	Antonio	Octavio	Manuel	Montse			
Person Mid Initials		A					
Person Email	agomezm@idibell.cat						
Person Affiliation	IDIBELL						
Person Address	PEBC, IDIBELL, Hospital Duran i Reynals Av. Gran Via s/n km, 2.7, L'Hospitalet de Llobregat, Barcelona, Spain						
Person Roles	submitter						
Protocol Name	P-GSE48559-1	P-GSE48559-5	P-GSE48559-6	P-GSE48559-2	P-GSE48559-3	P-GSE48559-4	P-GSE48559-7
Protocol Description	Images were quantified using Agilent Feature Extraction Software (ver. 10.7) ID_REF =  VALUE = Normalized signal intensity	Amount of nucleic acid labeled: 100ng. Commercial One-Color Microarray-Based Gene Expression Analysis” v.6.6 kit by following manufacturer instructions. Agilent manual G4140-90040 of September 2012. Amplification: by RNA polymerases.	Microarray: SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent microarray design ID 039494)	Not special additional treatment	Cells where growth on RPMI media with 10% FBS, 1% PS	Total mRNA where extrated using Trizol Reagent.	Scanned on an G2505C DNA microarray scanner (Agilent).
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	CELL LINE						
Experimental Factor Type	cell line						
Publication Title	MAX inactivation in small-cell lung cancer disrupts the MYC-SWI/SNF programs and is synthetic lethal with BRG1.						
Publication Author List	Romero OA, Torres-Diz M, Pros E, Savola S, Gomez A, Moran S, Saez C, Iwakawa R, Villanueva A, Montuenga LM, Kohno T, Yokota J, Sanchez-Cespedes M						
PubMed ID	24362264						
Publication DOI	10.1158/2159-8290.CD-13-0799						
Comment[SecondaryAccession]	GSE48559						
Comment[GEOReleaseDate]	2013-12-28						
Comment[ArrayExpressSubmissionDate]	2013-07-05						
Comment[GEOLastUpdateDate]	2013-12-29						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-48559.sdrf.txt