Comment[ArrayExpressAccession]	E-GEOD-48368
MAGE-TAB Version	1.1											
Public Release Date	2014-05-01
Investigation Title	Gene expression profile of PBMCs after intake of fish oil for seven weeks compared to high oleic sunflower oil											
Comment[Submitted Name]	Gene expression profile of PBMCs after intake of fish oil for seven weeks compared to high oleic sunflower oil
Experiment Description	Fish oil supplementation has been shown to alter gene expression of mononuclear cells both in vitro and in vivo. However, little is known about the total transcriptomic profile in healthy subjects after intake of fish oil compared to a control group. The objective was to examine the gene expression profile in peripheral blood mononuclear cells (PBMCs) in healthy subjects after intake of fish oil for seven weeks using whole-genome transcriptomic analysis. In a double-blinded randomized controlled study, healthy subjects received capsules containing either 8 g/d of fish oil (1.6 g/d EPA+DHA) (n=17) or 8 g/d of high oleic sunflower oil (n=19) for seven weeks. The results provide important information on how fish oil may modulate basic cellular processes involved in normal cell function and lymphocyte activation such as ER stress, cell cycle and apoptosis. The subjects were taking 16 capsules/d containing 8 g/d of either fish oil (FO) or high oleic sunflower oil (HOSO) for seven weeks. Subjects in the FO group received capsules containing 0.7 g/d EPA + 0.9 g/d DHA from cod liver oil (Gadidae sp., TINE EPADHA Oil 1200) provided by TINE SA (Oslo, Norway) and subjects in HOSO group received high oleic sunflower oil purchased from AarhusKarlshamn AB (Malmӧ, Sweden). Prior to the baseline visit (visit 2, wk 0), the subjects conducted a four-week washout period, where foods containing marine n-3 fatty acids were excluded from the diet. The first three weeks of the intervention period the subjects conducted a fully-controlled isocaloric diet, provided with all food and beverages at Akershus University College, Norway. During the last four weeks of the intervention the subjects returned to their habitual diet. Use of fish, fish products, marine n-3 enriched food or dietary supplements was not allowed during the entire study period of 11 weeks. The study was registered at www.clinicaltrial.gov (IDno. NCT01034423). The subjects met for four visits and blood samples for the transcriptome analyses were collected at wk 0, 3 and 7. After blood collection, PBMCs were isolated by using the BD Vacutainer Cell Preparation tubes according to the manufacturer's instructions (Becton, Dickinson and Company, NJ 07417, USA). Pellets were frozen and stored at -80o C for further RNA isolation.											
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl										
Person Last Name	Myhrstad	Myhrstad	Ulven	Günther	Ottestad	Holden	Ryeng	Borge	Kohler	Brønner	Thoresen	Holven
Person First Name	Mari	Mari	Stine	Clara	Inger	Marit	Einar	Grethe	Achim	Kirsti	Magne	Kirsten
Person Mid Initials		C	M	C				I		W		
Person Email	mari.myhrstad@hioa.no											
Person Affiliation	Oslo and Akershus University College											
Person Phone	+ 4791392176											
Person Address	Department of health, Oslo and Akershus University College, Postboks 4, St. Olavs plass, OSLO, Norway											
Person Roles	submitter											
Protocol Name	P-GSE48368-1	P-GSE48368-3	P-GSE48368-4	P-GSE48368-2	P-GSE48368-5							
Protocol Description	The data were normalised using quantile normalisation with IlluminaGUI in R. ID_REF =  VALUE = Log2 quantile-normalized signal intensity Detection Pval = 	Labelled extracts were prepared using Illumina TotalPrep RNA Amplification Kit (Illumina Inc, CA 92122, USA).	Standard Illumina hybridization protocol.	Total RNA was isolated from all PBMC samples using RNeasy mini kit (Qiagen) with lysis buffer added beta-mercaptoethanol according to the manufacturer's instructions, and stored at -80C. Quality control was performed with Agilent Bioanalyser.	Standard Illumina scanning protocol.							
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol							
Experimental Factor Name	INDIVIDUAL ID	TREATMENT	TIME									
Experimental Factor Type	individual id	treatment	time									
Comment[SecondaryAccession]	GSE48368											
Comment[GEOReleaseDate]	2014-05-01											
Comment[ArrayExpressSubmissionDate]	2013-06-27											
Comment[GEOLastUpdateDate]	2014-05-01											
Comment[AEExperimentType]	transcription profiling by array											
Comment[AdditionalFile:Data1]	GSE48368_non-normalized.txt											
SDRF File	E-GEOD-48368.sdrf.txt