Comment[ArrayExpressAccession]	E-GEOD-47543
MAGE-TAB Version	1.1				
Public Release Date	2013-05-31
Investigation Title	The physicochemistry of capped nanosilver predicts it biological activity in brain endothelial (RBEC4) cells				
Comment[Submitted Name]	The physicochemistry of capped nanosilver predicts it biological activity in brain endothelial (RBEC4) cells
Experiment Description	This study uses the RBEC4 cell model to examine if nanosilver of different sizes (10nm and 75nm) and cappings (citrate and polyvinylpyrrolidone(PVP)) affect its cellular permeability and genomic response. RBEC4 cells (95% confluent) were screeened for changes in gene expression after 18 hour exposure to 1.0 ppm of PVP or citrate coated nanosilver particles at 10 or 75 nanometer sizes.  Analysis indicated that the cellular transcription profile alter by nanosilver 10nm+PVP was distinct from it 75nm counterpart and both sizes of citrate-coated particles.				
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl			
Person Last Name	Ward	Veronesi	Chorley	Valant	Ward
Person First Name	William	Bellina	Brian	Beena	William
Person Email	ward.william@epa.gov				
Person Affiliation	United States EPA				
Person Phone	919-541-2317				
Person Fax	919-541-0674				
Person Address	NHEERL, United States EPA, 109 TW Alexander Dr, Durham, NC, USA				
Person Roles	submitter				
Protocol Name	P-GSE47543-1	P-GSE47543-3	P-GSE47543-4	P-GSE47543-2	P-GSE47543-5
Protocol Description	The data were normalised using quantile normalisation with no background subtraction in Illumina GenomeStudio (V.2011.1). ID_REF =  VALUE = quantile normalized with no background subtraction Detection Pval = 	Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification kit for Illumina arrays	Standard Illumina hybridization protocol	RNA was extracted with QIAGEN RNeasy mini kit with DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser and quantified by NanoDrop spectrophotometer.	Standard Illumina scanning protocol
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	EXPOSED TO				
Experimental Factor Type	exposed to				
Comment[SecondaryAccession]	GSE47543				
Comment[GEOReleaseDate]	2013-05-31				
Comment[ArrayExpressSubmissionDate]	2013-05-30				
Comment[GEOLastUpdateDate]	2013-06-04				
Comment[AEExperimentType]	transcription profiling by array				
Comment[AdditionalFile:Data1]	GSE47543_non_normalized.txt				
SDRF File	E-GEOD-47543.sdrf.txt