Comment[ArrayExpressAccession]	E-GEOD-46465
MAGE-TAB Version	1.1						
Public Release Date	2013-08-01
Investigation Title	Transcriptional responses of human colon and liver cancer cells to TCF inhibition.						
Comment[Submitted Name]	Transcriptional responses of human colon and liver cancer cells to TCF inhibition.
Experiment Description	Dysregulation of Wnt/TCF signaling is closely associated with cancers arising from the gastrointestinal tract, inlcluding colon cancer and liver cancer. The goal of this study is to understand the transcriptional programs underlying Wnt/TCF activation in gastrointestinal cancers. We examined the transcriptional responses to TCF inhibition in cultured human colon cancer cells and liver cancer cells that are characteristic of Wnt pathway activation. Human liver cancer cell line HepG2 and colon cancer cell line LS174T with or without expression of a dominant negative form of TCF4						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Mao	Wang	Mao				
Person First Name	Junhao	Jiayi	Junhao				
Person Email	junhao.mao@umassmed.edu						
Person Affiliation	University of Massachusetts Medical School						
Person Address	Cancer Biology, University of Massachusetts Medical School, 364 Plantation Street, LRB 417, Worcester, MA, USA						
Person Roles	submitter						
Protocol Name	P-GSE46465-1	P-GSE46465-5	P-GSE46465-6	P-GSE46465-2	P-GSE46465-3	P-GSE46465-4	P-GSE46465-7
Protocol Description	Data were analyzed by dChIP ID_REF =  VALUE = dChIP signaling intensity	300 ng of total RNA were labeled for each sample using the GeneChip WT cDNA Synthesis and Amplification Kit and WT Terminal Labeling Kit (Affymetrix, 900673 and 900671).	13.5 mg of fragmented biotinylated cRNA was fragmented and hybridized for 16 hr at 45C on human genechip 1.0 st arrays. Arrays were washed and stained in the Affymetrix Fluidics Station 450.	LS174T and HepG2 cells were transciently transfected with a DN-TCF4 GFP-expression vector using Lipofectamine 2000, and cells with high-level GFP expression were isolated 48 hours post transfection using flow cytometry.	LS174T and HepG2 cells were cultured in DMEM supplemented with 10% FBS. HepG2 cells were grown on cell cultured dishes coated with collegan I.	Total RNA was isolated using TRIZOL Reagent (Invitrogen, 15596-026) and purified by RNeasy Mini Kit (QIAGEN, 74104).	Arrays were scanned with the GeneChip Scanner 3000 7G
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	CELL LINE	EXPRESSION					
Experimental Factor Type	cell line	expression					
Comment[SecondaryAccession]	GSE46465						
Comment[GEOReleaseDate]	2013-08-01						
Comment[ArrayExpressSubmissionDate]	2013-04-29						
Comment[GEOLastUpdateDate]	2013-08-03						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-46465.sdrf.txt