Comment[ArrayExpressAccession]	E-GEOD-46267
MAGE-TAB Version	1.1							
Public Release Date	2013-12-09
Investigation Title	microRNAs involved in regulating embolic stroke recovery following spontaneous reperfusion [mRNA]							
Comment[Submitted Name]	microRNAs involved in regulating embolic stroke recovery following spontaneous reperfusion [mRNA]
Experiment Description	To date, miRNA and mRNA expression studies on cerebral ischemia in both human and animal models have focused mainly on acute phase of ischemic stroke. In this study, we present the roles played by microRNAs in the spontaneous recovery phases in cerebral ischemia using rodent stroke models. In this study presented here, Middle Cerebral Artery Occlusion stroke model was established by using embolus and the brain samples of stroke model were harvestd at 0hrs, 3hrs, 6hrs, 12hrs, 24hrs, 48hrs, 72hrs, 120hrs and 168hrs. RNAs were extracted from these samples and microRNA array and mRNA array were performed.							
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Jeyaseelan	Liu	Lim	Kaur	Sepramaniam	Armugam	Wong	Jeyaseelan
Person First Name	Kandiah	Fu	Kai	Prameet	Sugunavathi	Arunmozhiarasi	Peter	Kandiah
Person Mid Initials		J	Y				T	
Person Email	bchjeya@nus.edu.sg							
Person Affiliation	National University of Singapore							
Person Phone	+6565163248							
Person Fax	+6567791453							
Person Address	Biochemistry, National University of Singapore, 8 Medical Drive, Singapore, Singapore							
Person Roles	submitter							
Protocol Name	P-GSE46267-1	P-GSE46267-5	P-GSE46267-6	P-GSE46267-2	P-GSE46267-3	P-GSE46267-4	P-GSE46267-7	
Protocol Description	Differentially expressed genes were selected based on p value < 0.05, differential score > 20, average signal intensity > 100, and normalize to endo-control and baseline (Illumina Inc. GenomeStudio Version 1.8.0). ID_REF =  VALUE = normalized signal intensity	Biotin-labelled complementary RNA probes were synthesized with the Illumina TotalPrep RNA Amplification kit (Ambion).	Hybridization was performed in 15ul volume by using illumina hybridization kit.	no treatment	All animals were handled according to the Council for International Organisation of Medical Sciences on Animal Experimentation (World Health Organisation, Geneva, Switzerland) and the National University of Singapore (IACUC/NUS) guidelines for laboratory animals. The protocol was approved by the Committee on the Ethics of Animal Experiments of the National University of Singapore (Protocol Number: 081/09 and 025/11). All surgery was performed using ketamine/xylazine as anesthesia, and all efforts were made to minimize suffering.	Total RNA (+ miRNA) was extracted from brain tissues by a single-step method using Trizol reagent (Invitrogen, Life Technologies, USA) according to manufacturers’ protocol.	Data were scanned and analysed with the BeadStudio software (v.1.8.0).	
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol	
Publication Title	microRNAs Involved in Regulating Spontaneous Recovery in Embolic Stroke Model.							
Publication Author List	Liu FJ, Lim KY, Kaur P, Sepramaniam S, Armugam A, Wong PT, Jeyaseelan K							
PubMed ID	23823624							
Publication DOI	10.1371/journal.pone.0066393							
Comment[SecondaryAccession]	GSE46267							
Comment[GEOReleaseDate]	2013-12-09							
Comment[ArrayExpressSubmissionDate]	2013-04-22							
Comment[GEOLastUpdateDate]	2013-12-09							
Comment[AEExperimentType]	transcription profiling by array							
Comment[AdditionalFile:Data1]	GSE46267_non_normalized.txt							
SDRF File	E-GEOD-46267.sdrf.txt