Comment[ArrayExpressAccession]	E-GEOD-46244
MAGE-TAB Version	1.1						
Public Release Date	2013-09-01
Investigation Title	Expression data from wound skin taken at three time points post wounding						
Comment[Submitted Name]	Expression data from wound skin taken at three time points post wounding
Experiment Description	The full complement of hair follicles is generated during embryogenesis. Normally, no new hair is created after this time. Large full thickness skin excision wounding can result in the generation of new hair in the adult. Placodes can be observed following complete reepithelialization at wound day 14.  The events leading to hair neogenesis following wounding remain poorly understood. Late healing events (from wound day 10 to wound day 14) provide a possible window of induction for hair regeneration. We used microarrays to analyse changes in gene expression during late skin healing to provide candidates for factors involved in hair neogenesis following wounding. 6 week old C57Bl/6 mice received large full thickness skin excisions. Healing wound tissue was excised at wound day 10, 12 or 14 and analyzed for gene expression.						
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl					
Person Last Name	Gay	Gay	Cotsarelis				
Person First Name	Denise	Denise	George				
Person Email	denigay@mail.med.upenn.edu						
Person Affiliation	University of Pennsylvania						
Person Address	University of Pennsylvania, 422 Curie Blvd., Philadelphia, USA						
Person Roles	submitter						
Protocol Name	P-GSE46244-1	P-GSE46244-5	P-GSE46244-6	P-GSE46244-2	P-GSE46244-3	P-GSE46244-4	P-GSE46244-7
Protocol Description	CEL files were normalized with RMA in Partek Genomics Suite v6.6 ID_REF =  VALUE = log2-transformed RMA signal intensities	cRNA, amplified from 250ng total RNA, was converted to cDNA, fragmented, assessed by Bioanalyzer, and biotinylated by terminal transferase end labeling	5.5ug of labeled cDNA was added to Affymetrix hybridization cocktails, heated at 99ºC for 5 min and hybridized for 16 h at 45ºC to Mouse Gene 1.0ST GeneChips (Affymetrix Inc., Santa Clara CA)	Animals were sacrificed and wound tissue, both dermis and any overlying epidermis, was dissected out and flash frozen in liquid nitrogen. Care was taken to avoid surrounding unwounded skin and hair follicles at the wound edge. Wound tissue from three adult C57Bl/6 mice was combined for each time point analyzed.	Large 1.5cm2 full thickness excision wounds, removing both epidermis and underlying dermis, were created on the backs of adult C57Bl/6 mice. Following surgery, animals were housed in barrier facility conditions and observed daily for normal healing.	Total RNA was extracted using the Qiagen Rneasy Plus kit	Gene chips were scanned using a GeneChip 3000 7G scanner.
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	TIME						
Experimental Factor Type	time						
Comment[SecondaryAccession]	GSE46244						
Comment[GEOReleaseDate]	2013-09-01						
Comment[ArrayExpressSubmissionDate]	2013-04-21						
Comment[GEOLastUpdateDate]	2013-09-03						
Comment[AEExperimentType]	transcription profiling by array						
SDRF File	E-GEOD-46244.sdrf.txt