Comment[ArrayExpressAccession]	E-GEOD-45812
MAGE-TAB Version	1.1									
Public Release Date	2013-05-01
Investigation Title	Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex									
Comment[Submitted Name]	Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex
Experiment Description	This SuperSeries is composed of the SubSeries listed below. Refer to individual Series									
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl								
Person Last Name	Gurdziel									
Person First Name	Katherine									
Person Email	geo@ncbi.nlm.nih.gov									
Person Affiliation	University of Michigan									
Person Address	University of Michigan, 109 Zina Pitcher Place, Ann Arbor, USA									
Person Roles	submitter									
Protocol Name	P-GSE45812-1	P-GSE45812-2	P-GSE45812-8	P-GSE45812-5	P-GSE45812-9	P-GSE45812-6	P-GSE45812-3	P-GSE45812-4	P-GSE45812-10	P-GSE45812-7
Protocol Description	Microarray data were normalized using the robust multiarray average (RMA) algorithm (limma R package). ID_REF =  VALUE = normalized signal intensities	The non-normalized_data.txt file contains the preprocessed data (CT values) exported from the OpenArray ® Real-Time qPCR Analysis Software.  Data were analyzed with the Applied Biosystems DataAssist ™ Software. CT values normalized to U6 using the Applied Biosystems DataAssist ™ Software. Fold change (KO/WT) ID_REF =  VALUE = CT values normalized to U6	Megaplex ™ RT Primers were used to label the cDNA. Protocol specified in TaqMan(R) OpenArray(R) MicroRNA Panels User Guide June 2011.	standard Affymetrix protocol	n/a	standard Affymetrix protocol	Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.	Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.	n/a	standard Affymetrix protocol
Protocol Type	normalization data transformation protocol	normalization data transformation protocol	labelling protocol	labelling protocol	hybridization protocol	hybridization protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol	array scanning protocol
Experimental Factor Name	DEVELOPMENTAL STAGE	GENOTYPE	AGE							
Experimental Factor Type	developmental stage	genotype	age							
Publication Title	Dicer deficiency reveals microRNAs predicted to control gene expression in the developing adrenal cortex.									
Publication Author List	Krill KT, Gurdziel K, Heaton JH, Simon DP, Hammer GD									
PubMed ID	23518926									
Publication DOI	10.1210/me.2012-1331									
Comment[SecondaryAccession]	GSE45812									
Comment[GEOReleaseDate]	2013-05-01									
Comment[ArrayExpressSubmissionDate]	2013-04-05									
Comment[GEOLastUpdateDate]	2014-05-28									
Comment[AEExperimentType]	transcription profiling by array									
Comment[AEExperimentType]	transcription profiling by RT-PCR									
SDRF File	E-GEOD-45812.sdrf.txt