Comment[ArrayExpressAccession]	E-GEOD-45755
MAGE-TAB Version	1.1					
Public Release Date	2013-04-04
Investigation Title	Deletion of the NF-κB subunit p65/RelA in the hematopoietic compartment leads to defects in hematopoietic stem cell function.					
Comment[Submitted Name]	Deletion of the NF-κB subunit p65/RelA in the hematopoietic compartment leads to defects in hematopoietic stem cell function.
Experiment Description	Deletion of the NF-κB subunit p65/RelA in the hematopoietic compartment results in gene expression changes in lineage-Flk2-c-kit+Sca-1+ cells from mouse bone marrow. We analyzed lineage-Flk2-c-kit+Sca-1+ cells from the bone marrow of p65 wild-type or knockout mice using the Affymetrix Mouse Exon 1.0 ST platform. Array data was processed by Affymetrix Exon Array Computational Tool. No technical replicates were performed.					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Stein	Stein	Baldwin			
Person First Name	Sarah	Sarah	Albert			
Person Mid Initials			S			
Person Email	craychee@email.unc.edu					
Person Affiliation	University of North Carolina at Chapel Hill					
Person Phone	919-966-3884					
Person Fax	919-966-8212					
Person Address	Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 450 West Dr., Chapel Hill, NC, USA					
Person Roles	submitter					
Protocol Name	P-GSE45755-1	P-GSE45755-4	P-GSE45755-5	P-GSE45755-2	P-GSE45755-3	P-GSE45755-6
Protocol Description	RMA expression value derived from Expression Console software, and analysis was performed using Partek Genomics Suite. affymetrix-algorithm-name = rma-gene-default, affymetrix-algorithm-version = 1.0, program-name = Expression Console, program-version = 1.1.2800.28061 ID_REF =  VALUE = RMA-normalized	RNA was amplified and labeled using the Nugen Ovation Pico WTA System V2.	Samples were hybridized to Affymetrix Mouse Gene Chip 1.0 ST arrays and scanned at the UNC Affymetrix Core Facility.	Murine bone marrow lineage-Flk2-Sca-1+c-kit+ cells were sorted directly into buffer RLT (Qiagen) with 1% β-Mercaptoethanol.	RNA was extracted with the RNEasy Mini Kit (Qiagen).	Array scanning was performed according to the manufacturer's instruction (Affymetrix).
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	VARIATION					
Experimental Factor Type	variation					
Comment[SecondaryAccession]	GSE45755					
Comment[GEOReleaseDate]	2013-04-04					
Comment[ArrayExpressSubmissionDate]	2013-04-03					
Comment[GEOLastUpdateDate]	2013-04-05					
Comment[AEExperimentType]	transcription profiling by array					
SDRF File	E-GEOD-45755.sdrf.txt