Comment[ArrayExpressAccession]	E-GEOD-45500
MAGE-TAB Version	1.1							
Public Release Date	2013-10-29
Investigation Title	Gene expression profiles of unfertilized eggs of Ciona intestinalis							
Comment[Submitted Name]	Gene expression profiles of unfertilized eggs of Ciona intestinalis
Experiment Description	The transcriptome of unfertilized eggs of Ciona intestinalis was determined with microarrays. To obtain gene expression profiles of unfertilized eggs, we took 14 biological replicates. For the first through fourth replicates, we also took a technical duplicate. Gene expression profiles of the 8-, 16-, 32-cell embryos were obtained with embryos from the third to fifth animals.							
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Satou	Matsuoka	Satou					
Person First Name	Yutaka	Terumi	Yutaka					
Person Email	yutaka@ascidian.zool.kyoto-u.ac.jp							
Person Affiliation	Kyoto University							
Person Phone	+81757534095							
Person Address	Department of zoology, Graduate School of Science, Kyoto University, Kitashirakawa Oiwake-tyo, Sakyo-ku, Kyoto, Kyoto, Japan							
Person Roles	submitter							
Protocol Name	P-GSE45500-2	P-GSE45500-1	P-GSE45500-6	P-GSE45500-7	P-GSE45500-3	P-GSE45500-4	P-GSE45500-5	P-GSE45500-8
Protocol Description	The scanned images were quantified by Feature Extraction Software ver.10.7( Agilent Technologies) using default parameters. ID_REF =  VALUE = Normalized signal intensity	The scanned images were quantified by Feature Extraction Software ver.10.7( Agilent Technologies) using default parameters. ID_REF =  VALUE = Normalized signal intensity (gProcessedSignal)	50ng total RNA was labeled using Low Input Quick Amp Labeling Kit (Agilent) according to the manufacture’s instructions (www.agilent.com/).	Hybridization and washing were performed using GE Hybridization kit (Agilent) and GE Washing Buffer (Agilent) according to the manufacture’s instructions.	Unfertilized eggs of C.intestinalis were obtained by dissection from one individual and dechorionized. After insemination, fertilized eggs were incubated in Millipore-filtered seawater at 18℃, and 8-, 16- and 32-cell embryos were sampled.	Wild Ciona intestinalis adults were used.	The total RNA was extracted using RNeasy Mini Kit (QIAGEN) according to the manufacture’s protocols. The quality of extracted RNA was analyzed on the Agilent 2100 Bioanalyzer (Agilent Technologies) using Agilent RNA 6000 Nano Kit (Agilent). The RNA yield was measured with Quant-iT RNA Assay Kit (Invitrogen).	The slides after washing were scanned on an Agilent G2505C Microarray Scanner (Agilent Technologies) using default settings. The scanned file option, TIFF file dynamic range was set to 20-bit.
Protocol Type	normalization data transformation protocol	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	growth protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	DEVELOPMENTAL STAGE	INDIVIDUAL						
Experimental Factor Type	developmental stage	individual						
Publication Title	Transcriptome dynamics in early embryos of the ascidian, Ciona intestinalis.							
Publication Author List	Matsuoka T, Ikeda T, Fujimaki K, Satou Y							
PubMed ID	24120375							
Publication DOI	10.1016/j.ydbio.2013.10.003							
Comment[SecondaryAccession]	GSE45500							
Comment[GEOReleaseDate]	2013-10-29							
Comment[ArrayExpressSubmissionDate]	2013-03-26							
Comment[GEOLastUpdateDate]	2013-10-29							
Comment[AEExperimentType]	transcription profiling by array							
SDRF File	E-GEOD-45500.sdrf.txt