Comment[ArrayExpressAccession]	E-GEOD-45328
MAGE-TAB Version	1.1																											
Public Release Date	2013-03-21
Investigation Title	Inactivation of TP53 correlates with disease progression and low miR-34a expression in previously treated chronic lymphocytic leukemia patients																											
Comment[Submitted Name]	Inactivation of TP53 correlates with disease progression and low miR-34a expression in previously treated chronic lymphocytic leukemia patients
Experiment Description	Micro-RNA expression data of CD19 selected B-cells from previously treated and relapsed chronic lymphocytic leukemia patients. We aimed to correlate miR-34a with TP53 mutation status and del17p status. CD19 B-cells from previously treated and relapsed chronic lymphocytic leukemia patients were selected for RNA extraction and hybridization on Affymetrix microarrays.																											
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl																										
Person Last Name	Palermo	Dufour	Palermo	Zellmeier	Mellert	Duchateau-Nguyen	Schneider	Benthaus	Kakadia	Spiekermann	Hiddemann	Braess	Truong	Patten	Wu	Lohmann	Dornan	GuhaThakurta	Yeh	Salogub	Solal-Celigny	Dmoszynska	Robak	Montillo	Catalano	Geisler	Weisser	Bohlander
Person First Name	Giuseppe	Annika	Giuseppe	Evelyn	Gudrun	Guillemette	Stephanie	Tobias	Purvi	Karsten	Wolfgang	Jan	Sim	Nancy	Lin	Sabine	David	Debraj	Ru-Fang	Galina	Philippe	Anna	Tadeusz	Marco	John	Christian	Martin	Stephan
Person Mid Initials									M																	H		K
Person Email	giuseppe.palermo@roche.com																											
Person Affiliation	F. Hoffman-La Roche Ltd.																											
Person Address	F. Hoffman-La Roche Ltd., wettsteinallee 76, Basel, Switzerland																											
Person Roles	submitter																											
Protocol Name	P-GSE45328-1	P-GSE45328-5	P-GSE45328-4	P-GSE45328-2	P-GSE45328-3	P-GSE45328-6																						
Protocol Description	ID_REF =  VALUE = log2 RMA signal	Imaging and signal extraction were performed according to Affymetrix-recommended procedures. GCOS 1.3 was used	Hybridization was performed according to Affymetrix-recommended procedures, except that the 20X GeneChip Eukaryotic Hybridization Controls were omitted from the hybridization.	RNA of CD19 selected B-cells was isolated using Ambion miRVANA total RNA extraction kit	The 3’ ends of RNA molecules in total RNA samples (400ng total RNA per sample) were labeled with biotin according to the company’s standard protocol	The probe intensities of miRNA arrays were background-subtracted, quantile normalized and summarized using the RMA method. All data were log2-transformed																						
Protocol Type	bioassay_data_transformation	image_aquisition	hybridization	nucleic_acid_extraction	labeling	feature_extraction																						
Experimental Factor Name	MULTIPLE P53 MUTATION	P53 MUTATION	DOMINANT P53 MUTATION	DEL17P																								
Experimental Factor Type	multiple p53 mutation	p53 mutation	dominant p53 mutation	del17p																								
Comment[SecondaryAccession]	GSE45328																											
Comment[GEOReleaseDate]	2013-03-21																											
Comment[ArrayExpressSubmissionDate]	2013-03-20																											
Comment[GEOLastUpdateDate]	2013-03-22																											
Comment[AEExperimentType]	transcription profiling by array																											
SDRF File	E-GEOD-45328.sdrf.txt