Comment[ArrayExpressAccession]	E-GEOD-44926
MAGE-TAB Version	1.1							
Public Release Date	2013-10-17
Investigation Title	Global gene expression analysis of vascular progenitors differentiated from human embryonic stem cells or induced pluripotent stem cells, corresponding donor starting cells, and mature human endothelial cells							
Comment[Submitted Name]	Global gene expression analysis of vascular progenitors differentiated from human embryonic stem cells or induced pluripotent stem cells, corresponding donor starting cells, and mature human endothelial cells
Experiment Description	Global gene expression analysis of FACS-purified CD31+CD146+ vascular progenitors (VP) derived from (a) human embryonic stem cells (VP-hESC), (b) adult fibroblast derived induced pluripotent stem cells (VP-AdF-iPSC), (c) stromal primed cord blood CD34+ myeloid progenitor derived iPSC (VP-sp-CB-iPSC), (d) corresponding starting fibroblasts and myeloid progenitors, (e) human umbilical vein endothelial cells, and (f) human dermal microvascular endothelial cells. Total RNA was harvested from (a) adult fibroblasts, (b) human myeloid progenitors: Non-nucloefected CD34+ CB cells that were expanded with growth factors from Day -3 until Day 0 and harvested, (c) FACS-purified CD31+CD146+ vascular progenitors (VP) that were differentiated from human embryonic stem cells (hESC), (d) VP differentiated from low passage stromal-primed episomal iPSC derived from growth-factor activated cord blood myeloid progenitors, (e) VP differentiated from iPSC derived from adult fibroblasts, (f) human umbilical vein endothelial cells, and (g) human dermal microvascular endothelial cells.. Illumina HumanHT-12 V4.0 expression beadchips were used for all analyses in this series. Three or independent samples of each sample type were each run on individual microarrays.							
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl						
Person Last Name	Zambidis	Park	Zimmerlin	Huo	Talbot	Aguilar	Yu	Zambidis
Person First Name	Elias	Tea	Ludovic	Jeffrey	C	Jack	Wayne	Elias
Person Mid Initials	Thomas	S		S	C			T
Person Email	geo@ncbi.nlm.nih.gov							
Person Affiliation	Johns Hopkins School of Medicine							
Person Address	Oncology, Johns Hopkins School of Medicine, 733 N. Broadway, Baltimore, MD, USA							
Person Roles	submitter							
Protocol Name	P-GSE44926-1	P-GSE44926-3	P-GSE44926-4	P-GSE44926-2	P-GSE44926-5			
Protocol Description	Data were extracted with Gene Expression Module 1.0.6 in GenomeStudio 1.0.2 and signal intensities from multiple chips comprising this single series were quantile normalized without background subtraction to obtain normalized data as deposited. ID_REF =  VALUE = normalized signal intensity	500 ng total RNA from each sample was amplified and labeled using the Illumina TotalPrep RNA Amplification Kit (AMIL1791, Ambion, Austin, TX) as described in the instruction manual.	For array assay, 750 ng biotin-labeled cRNA was combined with hybridization buffer and hybridized to the array at 58°C for 16-20 hours. After hybridization, the hybridization cartridge was disassembled and the array was washed with buffer at 55C and blocked at room temperature. Bound biotinylated cRNA was stained with streptavidin-Cy3 and then washed. Illumina HumanHT-12 V4.0 expression beadchips were used for all analyses in this series.	Total RNA was prepared as described in the RNeasy Mini Kit (Qiagen) with on-column DNase I digestion. RNA quality was assessed by Nanodrop-1000 spectrometer for OD260/280 and OD260/230 ratio and Bioanalyzer (Agilent Technologies).	Fluorescent signals were obtained by scanning with the iScan System.			
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol			
Experimental Factor Name	SAMPLE TYPE	CELL TYPE						
Experimental Factor Type	sample type	cell type						
Comment[SecondaryAccession]	GSE44926							
Comment[GEOReleaseDate]	2013-10-17							
Comment[ArrayExpressSubmissionDate]	2013-03-06							
Comment[GEOLastUpdateDate]	2013-10-17							
Comment[AEExperimentType]	transcription profiling by array							
Comment[AdditionalFile:Data1]	GSE44926_non_normalized.txt							
SDRF File	E-GEOD-44926.sdrf.txt