Comment[ArrayExpressAccession] E-GEOD-44139 MAGE-TAB Version 1.1 Public Release Date 2014-10-01 Investigation Title miRNAs were significantly regulated by myocardin and ERM-NM-1 Comment[Submitted Name] miRNAs were significantly regulated by myocardin and ERM-NM-1 Experiment Description Using microarray, we compared miRNAs expression profiles of MDA-MB-231 cells transfected with myocardin and empty vector (pcDNA3.1) and found that 25 miRNAs were significantly changed in myocardin-transfected groups (17 up-regulated and 9 down-regulated miRNAs). Moreover, we showed that 18 of 25 miRNAs significantly regulated by myocardin were inhibited by ERM-NM-1 in MDA-MB-231 cells. In addition,through a microarray approach, we identify the subset of miRNAs modulated by ERM-NM-1 in MDA-MB-231 cells. Our results determined that ERM-NM-1 may function as tumor-promoter through down-regulating expression of 3 miRNAs (miR-26b, miR-146a and miR-331-3p) in MDA-MB-231 cells. There are 4 samples in this experiment (pcDNA3.1-transfected, myocardin-transfected, ERM-NM-1-transfected, myocardin plus ERM-NM-1 co-transfecteded groups) and each sample was replicated 3 times. The vector-pcDNA3.1 was used as control. Term Source Name ArrayExpress EFO Term Source File http://www.ebi.ac.uk/arrayexpress/ http://www.ebi.ac.uk/efo/efo.owl Person Last Name lu Lu Wang Liao Zhang Person First Name da Dalin Nan Xinghua Tongcun Person Mid Initials lin Person Email 468891883@qq.com Person Affiliation Wuhan university of science and technology Person Address Wuhan university of science and technology, Wuhan university of science and tecnology, Wuhan, China Person Roles submitter Protocol Name P-GSE44139-1 P-GSE44139-5 P-GSE44139-6 P-GSE44139-2 P-GSE44139-3 P-GSE44139-4 P-GSE44139-7 Protocol Description The data were analyzed with PARTEK genomic suit 6.4 using RMA normalization ID_REF = VALUE = log2 RMA Biotinylated microRNA were prepared according to the standard Affymetrix protocol from 1000ng total RNA (AffymetrixM-. FlashTagM-bM-^DM-" Biotin HSR RNA Labeling Kits). Following fragmentation, 1 ug RNA were hybridized for 16 hr at 48C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450. MDA-MB-231 breast carcinoma cells were separately transfected with myocardin, ERM-NM-1M-oM-