Comment[ArrayExpressAccession]	E-GEOD-44126
MAGE-TAB Version	1.1					
Public Release Date	2013-08-05
Investigation Title	Expression data from Human CD40L+ Tcells					
Comment[Submitted Name]	Expression data from Human CD40L+ Tcells
Experiment Description	To assess the nature of CD8+CD40L+ memory Tcells, we compared the gene expression to CD8+CD40L- and CD4+ counterparts, and found similarities in expression of genes encoding cytokines PBMCs were isolated from five healthy donors. The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort					
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl				
Person Last Name	Stervbo	Frentsch	Stark			
Person First Name	Ulrik	Marco	Regina			
Person Email	ulrik.stervbo@gmail.com					
Person Affiliation	BCRT					
Person Address	BCRT, FM-CM-6hrer StraM-CM-^_e 15, Berlin, Germany					
Person Roles	submitter					
Protocol Name	P-GSE44126-1	P-GSE44126-4	P-GSE44126-5	P-GSE44126-2	P-GSE44126-3	P-GSE44126-6
Protocol Description	Chip data were analyzed with GeneChip Operating Software (GCOS 1.1; Affymetrix) default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200 ID_REF =  VALUE = Log2 GC-RMA	Biotinylated cRNA were prepared from 3-5 ug total RNA according to the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)	15 M-5g fragmented cRNA was hybridized to the Affymetrix HG-U133Plus2 gene chip in a Hybridization Oven 640, washed and stained in the Fluidics Station 400 (Affymetrix), according to procedure 2 described in the technical manual 'GeneChip Expression Analysis' (Affymetrix, Santa Clara, CA)	PBMCs were isolated by Ficoll gradient from buffy coat. CD4+ and CD8+ T cells were isolated via MACS, The cells were stimulated with PMA/Ionomycin for 6 h, and subsequently sorted into the CD4+CD45RA-CD40L+, CD8+CD45RA-CD40L-, CD8+CD45RA-CD40L+ populations by FACS-sort	Trizol extraction of total RNA was performed according to the manufacturer's instructions.	The arrays were scanned with an Affymetrix GeneChip Scanner 3000
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	sample treatment protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	CELL POPULATION	SEX				
Experimental Factor Type	cell population	sex				
Publication Title	CD40L expression permits CD8+ T cells to execute immunologic helper functions.					
Publication Author List	Frentsch M, Stark R, Matzmohr N, Meier S, Durlanik S, Schulz AR, Stervbo U, JM-oM-?M-=rchott K, Gebhardt F, Heine G, Reuter MA, Betts MR, Busch D, Thiel A					
PubMed ID	23719298					
Publication DOI	10.1182/blood-2013-02-483586					
Comment[SecondaryAccession]	GSE44126					
Comment[GEOReleaseDate]	2013-08-05					
Comment[ArrayExpressSubmissionDate]	2013-02-06					
Comment[GEOLastUpdateDate]	2013-08-05					
Comment[AEExperimentType]	transcription profiling by array					
Comment[AdditionalFile:Data1]	GSE44126_list_of_interesting_genes.txt					
SDRF File	E-GEOD-44126.sdrf.txt