Comment[ArrayExpressAccession]	E-GEOD-44108
MAGE-TAB Version	1.1											
Public Release Date	2013-02-07
Investigation Title	Cardiac phase-targeted dynamic load on left ventricle differentially regulates phase-sensitive gene expressions and pathway activation											
Comment[Submitted Name]	Cardiac phase-targeted dynamic load on left ventricle differentially regulates phase-sensitive gene expressions and pathway activation
Experiment Description	Each heart was assigned to one of the following three conditions: phasic systolic overload (SO), phasic diastolic overload (DO), and no loading throughout the cardiac cycle (control; CN). We created SO by isovolumic contraction (peak systolic LVP > 170 mmHg) at a constant diastolic volume with end-diastolic pressure (EDP) equals 0 mmHg. For DO, we increased LVV in diastole so that EDP reached 40 mmHg, and unloaded quickly in systole to minimize LVP generation. At the end of 3 hours, the myocardium from each heart was collected for subsequent analyses. rat heart subjected to phasic systolic overload and diastolic overload											
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl										
Person Last Name	ide	Onitsuka	Ide	Arai	Hata	Murayama	Hosokawa	Sakamoto	Tobushi	Sakamoto	Fujino	Sunagawa
Person First Name	tomomi	Ken	Tomomi	Shinobu	Yuko	Yoshinori	Kazuya	Takafumi	Tomoyuki	Kazuo	Takeo	Kenji
Person Email	tomomi_i@cardiol.med.kyushu-u.ac.jp											
Person Affiliation	KYUSHU UNIVERSITY 											
Person Phone	+81-92-642-5360 											
Person Fax	+81-92-642-5374											
Person Address	GRADUATE SCHOOL OF MEDICAL SCIENCES, KYUSHU UNIVERSITY , 3-1-1 Maidashi, Higashi-ku, Fukuoka, Fukuoka, Japan											
Person Roles	submitter											
Protocol Name	P-GSE44108-1	P-GSE44108-3	P-GSE44108-4	P-GSE44108-2	P-GSE44108-5							
Protocol Description	The data were normalised using quantile normalisation with IlluminaGUI in R ID_REF =  VALUE = quantile normalized Detection Pval = 	Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays	Standard Illumina hybridization protocol	RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.	Standard Illumina scanning protocol							
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol							
Experimental Factor Name	TREATMENT											
Experimental Factor Type	treatment											
Publication Title	Cardiac phase-targeted dynamic load on left ventricle differentially regulates phase-sensitive gene expressions and pathway activation.											
Publication Author List	Onitsuka K, Ide T, Arai S, Hata Y, Murayama Y, Hosokawa K, Sakamoto T, Tobushi T, Sakamoto K, Fujino T, Sunagawa K											
PubMed ID	24004468											
Publication DOI	10.1016/j.yjmcc.2013.08.008											
Comment[SecondaryAccession]	GSE44108											
Comment[GEOReleaseDate]	2013-02-07											
Comment[ArrayExpressSubmissionDate]	2013-02-06											
Comment[GEOLastUpdateDate]	2013-06-18											
Comment[AEExperimentType]	transcription profiling by array											
Comment[AdditionalFile:Data1]	GSE44108_non-normalized.txt											
SDRF File	E-GEOD-44108.sdrf.txt