Comment[ArrayExpressAccession]	E-GEOD-44070
MAGE-TAB Version	1.1				
Public Release Date	2013-02-05
Investigation Title	Genome-wide analysis of gene expression profile of Intestinal (COLON) Tumors from APCmin/+/VP16LXRa vs APCmin/+/VP16				
Comment[Submitted Name]	Genome-wide analysis of gene expression profile of Intestinal (COLON) Tumors from APCmin/+/VP16LXRa vs APCmin/+/VP16
Experiment Description	Changes in gene expression profile of intestinal (COLON) Tumors from APCmin/+/VP16LXRa vs APCmin/+/VP16.  The hypothesis tested in the present study was that LXRa overexpression influence cancer growth modulating lipid metabolism in cancer cells. Results provide the information that LXRa induces expression of genes encoding proteins able to regulate cholesterol efflux. Total RNA obtained from colonic tumors from APCmin/+/VP16LXRa mice was compared to total RNA extracted from APCmin/+/VP16 mice.				
Term Source Name	ArrayExpress	EFO
Term Source File	http://www.ebi.ac.uk/arrayexpress/	http://www.ebi.ac.uk/efo/efo.owl			
Person Last Name	Moschetta	Bovenga	Andria		
Person First Name	Antonio	Fabiola	D'Orazio		
Person Email	geo@ncbi.nlm.nih.gov				
Person Affiliation	University of Bari & Consorzio Mario Negri Sud				
Person Address	University of Bari & Consorzio Mario Negri Sud, Via Nazionale 8A, Santa Maria Imbaro, Italy				
Person Roles	submitter				
Protocol Name	P-GSE44070-1	P-GSE44070-3	P-GSE44070-4	P-GSE44070-2	P-GSE44070-5
Protocol Description	The data were normalised using quantile normalization with Illumina Genome Studio Software; items with detection p values >0.001 or AVG Signal <100 in both groups were excluded. ID_REF =  VALUE = quantile normalized signal Detection Pval = 	Biotinylated cRNA were prepared with the Ambion Illumina® TotalPrepTM RNA Amplification Kit	Standard Illumina hybridization protocol	RNA was extracted with QIAzol reagent, following manufacturer's instructions. Quality control was performed with Biorad Experion.	Standard Illumina scanning protocol
Protocol Type	normalization data transformation protocol	labelling protocol	hybridization protocol	nucleic acid extraction protocol	array scanning protocol
Experimental Factor Name	GENOTYPE				
Experimental Factor Type	genotype				
Publication Title	Liver X receptors inhibit proliferation of human colorectal cancer cells and growth of intestinal tumors in mice.				
Publication Author List	Lo Sasso G, Bovenga F, Murzilli S, Salvatore L, Di Tullio G, Martelli N, D'Orazio A, Rainaldi S, Vacca M, Mangia A, Palasciano G, Moschetta A				
PubMed ID	23419360				
Publication DOI	10.1053/j.gastro.2013.02.005				
Comment[SecondaryAccession]	GSE44070				
Comment[GEOReleaseDate]	2013-02-05				
Comment[ArrayExpressSubmissionDate]	2013-02-05				
Comment[GEOLastUpdateDate]	2013-12-24				
Comment[AEExperimentType]	transcription profiling by array				
Comment[AdditionalFile:Data1]	GSE44070_non-normalized_data.txt				
SDRF File	E-GEOD-44070.sdrf.txt